Isolation and Characterization of Expression of Eimeria tenella (E. Tenella) Sporozoite Membrane Protein As Recombinant Principle For Production of Material Subunit Vaccine of Chicken Coccidiosis
Over the past few decades control of infectious disease in humans and animals has largely relied on the use antibiotics and another chemical agents. In contrast, livestock industry now faced with the restriction of the use of antibiotics and chemical agents for original animal products. The conse...
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| Main Author: | |
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| Format: | Article PeerReviewed |
| Language: | English English |
| Published: |
AUP
2011
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/55967/1/Peereview.m.yunus8.pdf http://repository.unair.ac.id/55967/2/Karil008.pdf http://repository.unair.ac.id/55967/ |
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| Institution: | Universitas Airlangga |
| Language: | English English |
| Summary: | Over the past few decades control of infectious disease in humans and animals has
largely relied on the use antibiotics and another chemical agents. In contrast, livestock industry
now faced with the restriction of the use of antibiotics and chemical agents for original animal
products. The consequence of this withdrawal for the poultry industry in Indonesia is
reduction of productivity due to an increased incidence of infection by certain pathogens. In
order to overcome these restrictions, the use of vaccine to control infectious disease is more
expectant to avoid dependence upon the use of antibiotic and other chemical agents. Moreover
the use of vaccine is more natural, safe, effective and effecient to enhance resistance and to
obstruct the development of infection agent. One of vaccine materials has being explorated and
it has good prospected is recombinant vaccine of protein membrane of sporozoite that can be
developed, manipulated artificial producible and applicable. In order to realize that principal
necessary performed isolation and characterization of protein membrane of E. tenella sporozoite
by extraction of protein membrane of E. tenella sporozoite using SDS-PAGE and Dot Blot.
Briefly, Isolation and identification of sporozoites membrane protein of E. tenella by SDS-PAGE
was conformity with characterization by immunoblotting (Dot Blot) for protein with molecule
weight of 17 kDa.
sporozoite membrane protein, E. tenella |
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