The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers

• Main Authors: D.M. Hariyadi, Y. Ma, Y. Wang, T. Bostrom, J. Malouf, M.S. Turner, B. Bhandari, A.G.A. Coombes
• Format: Article PeerReviewed
• Language: English; Indonesian
• Published: Elsevier 2014
• Subjects: RS Pharmacy and materia medica; RS200-201 Pharmaceutical dosage forms
• Online Access: http://repository.unair.ac.id/56080/2/1-s2.0-S1773224714500299-main.pdf__tid%3D9f2295e0-1aab-11e7-82f3-00000aacb361%26acdnat%3D1491471187_cf6124e236dc05750d88afce30f26b74; http://repository.unair.ac.id/56080/1/2.%20Validasi%20dan%20Penilaian%20KI.pdf; http://repository.unair.ac.id/56080/; http://www.journals.elsevier.com/journal-of-drug-delivery-science-and-technology
• Institution: Universitas Airlangga

Oral administration of dry vaccine formulations is acknowledged to offer major clinical and logistical benefits by eliminating the cold chain required for liquid preparations. A model antigen, bovine serum albumin (BSA) was encapsulated in alginate microspheres using aerosolisation. Hydrated microspheres 25 to 65 µm in size with protein loading of 3.3 % w/w were obtained. Environmental scanning electron microscopy indicated a stabilizing effect of encapsulated protein on alginate hydrogels revealed by an increase in dehydration resistance. Freeze drying of alginate microspheres without use of a cryoprotectant resulted in fragmentation and subsequent rapid loss of the majority of the protein load in simulated intestinal fluid in 2 h, whereas intact microspheres were observed following freeze-drying of BSA-loaded microspheres in the presence of maltodextrin. BSA release from freeze-dried preparations was limited to less than 7 % in simulated gastric fluid over 2 h, while 90 % of the protein load was gradually released in simulated intestinal fluid over 10 h. SDS-PAGE analysis indicated that released BSA largely preserved its molecular weight. These findings demonstrate the potential for manufacturing freeze-dried oral vaccines using alginate microspheres.