VALIDASI METODE KROMATOGRAFI GAS DENGAN DETEKTOR IONISASI NYALA (KG-DIN) UNTUK PENETAPAN KADAR ETANOL DALAM LEGEN ATAU NIRA SIWALAN (Borassus flabellifer Linn.)
METHOD VALIDATION OF GAS CHROMATOGRAPHY WITH FLAME IONIZATION DETECTOR FOR DETERMINATION OF ETANOL IN LEGEN OR SIWALAN (Borassus flabellifer Linn.)SAP AdhadiAM Siwalan (Borassus flabellifer Linn.) sap or legen is one of the Indonesian traditional beverage.Siwalan sap during storage contains alc...
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | Indonesian Indonesian |
Published: |
2016
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Subjects: | |
Online Access: | http://repository.unair.ac.id/56250/1/FF.%20KF.%2045-16%20Adh%20v%20abstrak.pdf http://repository.unair.ac.id/56250/13/FF_KF_45-16_Adh_v.pdf http://repository.unair.ac.id/56250/ http://lib.unair.ac.id |
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Institution: | Universitas Airlangga |
Language: | Indonesian Indonesian |
Summary: | METHOD VALIDATION OF GAS CHROMATOGRAPHY WITH
FLAME IONIZATION DETECTOR FOR DETERMINATION OF
ETANOL IN LEGEN OR SIWALAN (Borassus flabellifer Linn.)SAP
AdhadiAM
Siwalan (Borassus flabellifer Linn.) sap or legen is one of the
Indonesian traditional beverage.Siwalan sap during storage contains alcohol
(ethanol) as the product of fermentation by enzimatic or microorganisms.
The amount of alcohol in alcoholic beverages can affects central nervous
system (CNS). The purpose of this research is obtaining validated method
of gas chromatography with flame ionization detector for determination of
etanol in siwalan sap. Gas chromatography Agilent 6890 Series GC system,
with a Flame ionzation Detector (FID) and a coloum HP-5 was used in this
study. Optimal condition were obtained by using temperature inlet and
detector of 250°C, whilst the temperature of oven programed at 55 – 120°C.
Split ratio was set at 50:1 and the injection volume was 1.0 μl.The method
was proved to be selective. Linearity showed linear respone (r = 0.9999)
while correlation coeffecient of the function (Vxo)was 2.4%over the range of
concentration used (between 0.025% to 2%). Accuracy of method was in
range of accepted limits (90– 108%), recovery was found to be in the
average of 97.12%, while the precision was 1.51%. The detection limit is
5.68 x 10-4%, and the quantification limit is 1.72 x 10-3%. The proposed
method was successfully applied for the analysis of ethanol in siwalan
sap.Siwalan sap was stored until five days in room temperature, extracted
by appropriate solvent, and analyzed with gas chromatography method.All
of them showed the profile of alcohol concentration. The result of
quantitative analysis of ethanol content in siwalan sap in five different days
of storage were 0.5694, 2.429, 4.357, 5.678, 5.894 and 6.292%.
Keyword: Borassus flabellifer Linn., siwalan sap, gas chromatography,
ethanol, validation method. |
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