The Influence Of Sodium Orthovanadate On The P53 And Caspase 3 Expressions In Beta Cells Diabetic Mice Model

• Main Authors: Sholihatil Hidayati, Junaidi Khotib, Suharjono
• Format: Article PeerReviewed
• Language: English; Indonesian
• Published: Elsevier 2016
• Subjects: R Medicine; RS Pharmacy and materia medica; RS1-441 Pharmacy and materia medica
• Online Access: http://repository.unair.ac.id/56671/1/12738-58177-2-PB.pdf; http://repository.unair.ac.id/56671/2/6.%20Bukti%20C-06%20Penilaian%20Karya%20Ilmiah.pdf; http://repository.unair.ac.id/56671/; https://innovareacademics.in/journals/index.php/ijpps/article/view/12738
• Institution: Universitas Airlangga

Objective: The present study was designed to investigate the influence of sodium orthovanadate (SOV) on the P53 and caspase 3 expressions in beta cells of alloxan-induced diabetic mice. Methods: Mice were divided into 5 groups i.e. (1) control group, (2) diabetic group and (3-5) SOV-treated diabetic groups at dose of 16, 32 and 64 mg/kgBW respectively. Diabetic mice model was induced by intraperitoneal administration of alloxan monohydrate at the dose of 200 mg/kgBW. Diabetic state was occurred on third day after alloxan injection and then started the treatment of SOV for 7 days. The pancreas was harvested on ten day after treatment and was stained using routine histology staining with haematoxylin-eosin (HE) and aldehyde fuchsin (AF) for morphological analysis and immunohistochemical approaches to observe the expressions of P53 and caspase 3 in pancreas. Results: Diabetic condition was shown by the increasing of fasting blood glucose levels from 59.1±11.2 mg/dl to 310.6±107.2 mg/dl on third day. Administration of SOV with dose 16, 32 and 64 mg/kgBW reduced the fasting blood glucose levels after 7 days treatment (p<0.05) at diabetic mice, respectively 303,0±126,8, 231,8±57,1 and 75,6±40,8 mg/dl. The results of histology staining showed that SOV reduced apoptosis in beta cells. Using immunohistochemical approaches, SOV might decreased the P53 and caspase 3 expressions in beta cells alloxan-induced diabetic mice (p<0.05).