Perbandingan Hasil Pemeriksaan Morfologi Spermatozoa Manusia Menggunakan Metode Pewarnaan Papanicolaou, Diff-Quik dan Safranin-Kristal Violet di RSUD dr. Soetomo Surabaya

• Main Author: Hengki Lukas, dr
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian
• Published: 2016
• Subjects: RA1-1270 Public aspects of medicine
• Online Access: http://repository.unair.ac.id/57255/1/TA.%2001-16%20Luk%20p%20abstrak.pdf; http://repository.unair.ac.id/57255/9/57255.compressed.pdf; http://repository.unair.ac.id/57255/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

OBJECTIVE: The aim of this study was to analyzed the differences between the methods of Papanicolaou, Safranin-Crystal violet and Diff-Quik stain and to find the alternative of gold standard stain. METHOD: The Design of research was obeservational analytic laboratory study, performed on patient who came to dr Soetomo hospital, Surabaya in the October 2015 – Februari 2016. Reasearch examined the morfologi spermatozoa and examined with three method: Papanicoloau, Safranin-Crystal violet, and Diff-Quik and then compare it. RESULTS : The results, a total of human sperm who sperm morfologi examined with Papanicolaou, safranin-Crystal violet and Diff-Quik for five month at dr Soetomo hospital from October 2015 to February 2016 as many 90 taken his sperm and checking sperm morfologi with Papanicolaou, Safranin-Crystal violet, and Diff-Quik. The results of statistical analysis found that there is significant between three method that we examine. In the category length and width there are significant different p < α between Diff-Quik, Safranin-Crystal Violet and Diff-Quik-Papanicolaou, whereas no significant different between Safranin-Crystal violet -Papanicolaou p > α. Similar result were shown by category vacuoles, acrossom, mid piece, and ERC. In the third method showed a significant different. CONCLUSION : Safranin-Crystal Violet stained as good as gold standard for analyzing morphology human spermatozoa such as head size, head shape, vacuoles, acrosomes, mid piece , principal piece and Excess Residual Cytoplasm. Based on that, Safranin – Crystal Violet could be one of the alternatives in staining methods. KEY WORD : Sperm morphology, Papanicolaou, Safranin-Crystal violet, Diff-Quik.