SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3

This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the reduce of citocrom c and caspase 3 expression in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment...

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Main Author: RINA PUJIASTUTI, 061314153010
Format: Theses and Dissertations NonPeerReviewed
Language:Indonesian
Indonesian
Published: 2016
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Online Access:http://repository.unair.ac.id/57624/1/1.%20ABSTRAK%20TBR%2009-16%20Puj%20s.pdf
http://repository.unair.ac.id/57624/2/2.%20FULLTEXT%20TBR%2009-16%20Puj%20s.pdf
http://repository.unair.ac.id/57624/
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Institution: Universitas Airlangga
Language: Indonesian
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spelling id-langga.576242017-05-23T18:29:25Z http://repository.unair.ac.id/57624/ SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 RINA PUJIASTUTI, 061314153010 TS2284-2288 Animal feeds and feed mills. Pet food industry This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the reduce of citocrom c and caspase 3 expression in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment by maturation media (TCM-199 that were added FSH 0,01 μg/ml, LH 0,01 μg/ml, BSA 3%) were added by ITS with diffrentiated doses. P0: ITS 0 μg/ml, P1: ITS 10 μg/ml, P2: ITS 15 μg/ml, P3: 20 μg/ml. Oocytes maturation were carried on 38.5 0C in incubator CO2 5% for 24 hours. The result supplementation ITS P0 (0 μg/ml) was significantly different (p<0,05) with P1(10 μg/ml), P2 (15 μg/ml) and P3(20 μg/ml) in reduced the expression of cythocorme c and caspase 3 in Cumulus Oocyte Complex (COC). Both expression of cythocrome c was significantly differen (p<0,05), at P2 (15 μg/ml) and P3(20 μg/ml). The conclusion of ITS supplementation experimental on maturation medium is be able to decreased expression of cytochrome c and caspase 3 in oocytes maturation. 2016 Thesis NonPeerReviewed text id http://repository.unair.ac.id/57624/1/1.%20ABSTRAK%20TBR%2009-16%20Puj%20s.pdf text id http://repository.unair.ac.id/57624/2/2.%20FULLTEXT%20TBR%2009-16%20Puj%20s.pdf RINA PUJIASTUTI, 061314153010 (2016) SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3. Thesis thesis, Universitas Airlangga. http://lib.unair.ac.id
institution Universitas Airlangga
building Universitas Airlangga Library
country Indonesia
collection UNAIR Repository
language Indonesian
Indonesian
topic TS2284-2288 Animal feeds and feed mills. Pet food industry
spellingShingle TS2284-2288 Animal feeds and feed mills. Pet food industry
RINA PUJIASTUTI, 061314153010
SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3
description This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the reduce of citocrom c and caspase 3 expression in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment by maturation media (TCM-199 that were added FSH 0,01 μg/ml, LH 0,01 μg/ml, BSA 3%) were added by ITS with diffrentiated doses. P0: ITS 0 μg/ml, P1: ITS 10 μg/ml, P2: ITS 15 μg/ml, P3: 20 μg/ml. Oocytes maturation were carried on 38.5 0C in incubator CO2 5% for 24 hours. The result supplementation ITS P0 (0 μg/ml) was significantly different (p<0,05) with P1(10 μg/ml), P2 (15 μg/ml) and P3(20 μg/ml) in reduced the expression of cythocorme c and caspase 3 in Cumulus Oocyte Complex (COC). Both expression of cythocrome c was significantly differen (p<0,05), at P2 (15 μg/ml) and P3(20 μg/ml). The conclusion of ITS supplementation experimental on maturation medium is be able to decreased expression of cytochrome c and caspase 3 in oocytes maturation.
format Theses and Dissertations
NonPeerReviewed
author RINA PUJIASTUTI, 061314153010
author_facet RINA PUJIASTUTI, 061314153010
author_sort RINA PUJIASTUTI, 061314153010
title SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3
title_short SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3
title_full SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3
title_fullStr SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3
title_full_unstemmed SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3
title_sort suplementasi insulin transferrin selenium pada maturasi in vitro cumulus oocyte complex terhadap ekspresi sitocrom-c dan ekspresi caspase 3
publishDate 2016
url http://repository.unair.ac.id/57624/1/1.%20ABSTRAK%20TBR%2009-16%20Puj%20s.pdf
http://repository.unair.ac.id/57624/2/2.%20FULLTEXT%20TBR%2009-16%20Puj%20s.pdf
http://repository.unair.ac.id/57624/
http://lib.unair.ac.id
_version_ 1681147612061761536