SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3
This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the reduce of citocrom c and caspase 3 expression in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment...
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id-langga.576242017-05-23T18:29:25Z http://repository.unair.ac.id/57624/ SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 RINA PUJIASTUTI, 061314153010 TS2284-2288 Animal feeds and feed mills. Pet food industry This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the reduce of citocrom c and caspase 3 expression in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment by maturation media (TCM-199 that were added FSH 0,01 μg/ml, LH 0,01 μg/ml, BSA 3%) were added by ITS with diffrentiated doses. P0: ITS 0 μg/ml, P1: ITS 10 μg/ml, P2: ITS 15 μg/ml, P3: 20 μg/ml. Oocytes maturation were carried on 38.5 0C in incubator CO2 5% for 24 hours. The result supplementation ITS P0 (0 μg/ml) was significantly different (p<0,05) with P1(10 μg/ml), P2 (15 μg/ml) and P3(20 μg/ml) in reduced the expression of cythocorme c and caspase 3 in Cumulus Oocyte Complex (COC). Both expression of cythocrome c was significantly differen (p<0,05), at P2 (15 μg/ml) and P3(20 μg/ml). The conclusion of ITS supplementation experimental on maturation medium is be able to decreased expression of cytochrome c and caspase 3 in oocytes maturation. 2016 Thesis NonPeerReviewed text id http://repository.unair.ac.id/57624/1/1.%20ABSTRAK%20TBR%2009-16%20Puj%20s.pdf text id http://repository.unair.ac.id/57624/2/2.%20FULLTEXT%20TBR%2009-16%20Puj%20s.pdf RINA PUJIASTUTI, 061314153010 (2016) SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3. Thesis thesis, Universitas Airlangga. http://lib.unair.ac.id |
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TS2284-2288 Animal feeds and feed mills. Pet food industry RINA PUJIASTUTI, 061314153010 SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 |
description |
This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the reduce of citocrom c and caspase 3 expression in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment by maturation media (TCM-199 that were added FSH 0,01 μg/ml, LH 0,01 μg/ml, BSA 3%) were added by ITS with diffrentiated doses. P0: ITS 0 μg/ml, P1: ITS 10 μg/ml, P2: ITS 15 μg/ml, P3: 20 μg/ml. Oocytes maturation were carried on 38.5 0C in incubator CO2 5% for 24 hours. The result supplementation ITS P0 (0 μg/ml) was significantly different (p<0,05) with P1(10 μg/ml), P2 (15 μg/ml) and P3(20 μg/ml) in reduced the expression of cythocorme c and caspase 3 in Cumulus Oocyte Complex (COC). Both expression of cythocrome c was significantly differen (p<0,05), at P2 (15 μg/ml) and P3(20 μg/ml). The conclusion of ITS supplementation experimental on maturation medium is be able to decreased expression of cytochrome c and caspase 3 in oocytes maturation. |
format |
Theses and Dissertations NonPeerReviewed |
author |
RINA PUJIASTUTI, 061314153010 |
author_facet |
RINA PUJIASTUTI, 061314153010 |
author_sort |
RINA PUJIASTUTI, 061314153010 |
title |
SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 |
title_short |
SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 |
title_full |
SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 |
title_fullStr |
SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 |
title_full_unstemmed |
SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3 |
title_sort |
suplementasi insulin transferrin selenium pada maturasi in vitro cumulus oocyte complex terhadap ekspresi sitocrom-c dan ekspresi caspase 3 |
publishDate |
2016 |
url |
http://repository.unair.ac.id/57624/1/1.%20ABSTRAK%20TBR%2009-16%20Puj%20s.pdf http://repository.unair.ac.id/57624/2/2.%20FULLTEXT%20TBR%2009-16%20Puj%20s.pdf http://repository.unair.ac.id/57624/ http://lib.unair.ac.id |
_version_ |
1681147612061761536 |