PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI PADA ANALISIS ANDROGRAFOLIDA DALAM BAHAN BAKU DAN TABLET FRAKSI ETIL ASETAT Andrographis paniculata .
Ethyl acetat fractions from Andrographis paniculata has been developed as phytopharmaceutical products. Andrographolide which is the major active compound of Andrographis paniculata was determined as marker compund of ethyl acetate fractions of Andrographis paniculata products. To ensure the qual...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/57831/13/KKB%20KK-2%20TF%2014_16%20Ihs%20p%20ABSTRAK.pdf http://repository.unair.ac.id/57831/14/KKB%20KK-2%20TF%2014_16%20Ihs%20p.pdf http://repository.unair.ac.id/57831/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Ethyl acetat fractions from Andrographis paniculata has been developed
as phytopharmaceutical products. Andrographolide which is the major active
compound of Andrographis paniculata was determined as marker compund of ethyl
acetate fractions of Andrographis paniculata products. To ensure the quality,
efficacy and safety of the phytopharmaceutical products, a simple and selective
analytical method becomes important for the determination of andrographolide in
both the raw materials and products.
The purpose of this study was to develop and validate a simple and
selective High Performance Liquid Chromatography (HPLC) for determination
andrographolide in raw material and tablet etil acetate fractions Andrographis
paniculata.
This method was performed using a RP-C18 Column (3.0 x 50 mm i.d.,
2.7μm parcticle size) as stationary phase, column temperature was maintained at
30⁰C, isocratic mobile phase of metanol : water (pH 3.05 with phosporic acid)
(50:50 v/v) mobile phase with flow rate of 0.3 ml/menit, injection volume 0,5μl and
detected at 228 nm.
The results showed that method was selective to separate andrographolide
peak from other component with good resolution, retention time andrographolide
was 2,5 minute. The data for calibration plots showed good linear relationship with
r2 = 0.9996 in the concentration range 50-1000 ppm. The limit of detection and
Quantification were found 4.89 ppm and 16.19 ppm, respectively. The recovery
method was found between 93.76 and 101.72% and the relative standard deviation
method was found between 1.60% and 2.39%. |
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