EMBRYO COLLECTION TOWARD DIFFERENT DOSES OF PMSG IN RATS
Technique for induction of super ovulation in livestock industry has been applied, particuiarly when large numbers of embryos are required for embryo transfer' The purpose of the research was collected of the embryos among group which it treated different doses of PMSG. All population of femal...
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| Main Author: | |
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| Format: | Book PeerReviewed |
| Language: | English Indonesian |
| Published: |
Faculty of Veterinary Medicine Universitas Airlangga
2013
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/59224/1/karil%2016%20%28embryo%20collection%20toward%29.pdf http://repository.unair.ac.id/59224/2/peerreview%20karil%2016%20%28embryo%20collection%29.pdf http://repository.unair.ac.id/59224/ |
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| Institution: | Universitas Airlangga |
| Language: | English Indonesian |
| Summary: | Technique for induction of super ovulation in livestock industry has been applied, particuiarly when large numbers of embryos are required for embryo transfer'
The purpose of the research was collected of the embryos among group which it treated different doses of PMSG. All population of female rats were divided into three
groups consist of 10 animals each group. Each animal were iniected intra peritoneal at volume 0.1 cc. Doses of PMSG (lntergonan@ - Vemie) were 0 IU, 10 lU, and 20 IU,
respectively, on the day minus two. Therefore, all animals were iniected intra peritoneal on day zero through 10 lU HCG (Chorulon@ - Interved). On the day four, all
mated females were sacrificed and the uterus was taken out. A blunt 21 gauge needle was inserted through the cervic into the lumen of uteri. Then the Fallopian tubes were
cut off. Embryo collection were recovered through flushing 1cc Phosphate Buffer Solution (PBSI add with 200lo Fetal Calf Serum (FCSI supplemented. The treated group
administered dose 20 lU PMSG tend to increased number of eggs super ovulated On the similar way, the treated group administered dose 10 lU PMSG tend to increased too.
However, there was no significantly difference between both 10 IU PMSG group and 20 lu PMSG according to the number of mated rats and average of embryos each rats on
P>0.01. On the treated rats in both PMSG doses 10 IU and 20 IU, a number of embryos were found degenerated at the time of observation. The degenerated ovum was shown uncompacted or Ioss blastomer cells in inside ofthe zona pellucida. These undoubtedly caused by the high degree of fertilization failure, This research suggested that the dose of PMSG play the important role as a source of variation in embryo collection. |
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