Single Cell Sexing Using a Part of Metal Razor Blade of Bovine Embryos Through Male Specific Repetitive DNA
The development of quick and sensitive methods for sexing of bovine embryos in early stage would be considerable in economic advantages. Polymerase Chain Reaction (PCR) were used for amplification of DNA sequenced derived from the male sex chromosome. The sexed method was allows to removed single ce...
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| Main Author: | |
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| Format: | Article PeerReviewed |
| Language: | English Indonesian English |
| Published: |
IDOSI Publications
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/59276/1/11.pdf http://repository.unair.ac.id/59276/2/peerreview%20karil%2026%20%28single%20cell%20sexing%29.pdf http://repository.unair.ac.id/59276/3/karil%2026%20%28single%20cell%20sexing%20using%29.pdf http://repository.unair.ac.id/59276/ https://www.idosi.org/gv/gv16(1)16/11.pdf |
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| Institution: | Universitas Airlangga |
| Language: | English Indonesian English |
| Summary: | The development of quick and sensitive methods for sexing of bovine embryos in early stage would be considerable in economic advantages. Polymerase Chain Reaction (PCR) were used for amplification of DNA sequenced derived from the male sex chromosome. The sexed method was allows to removed single cell biopsy using a part of metal razor blade from bovine embryos in less than two minutes without compromise developmental potential. The embryo biopsy can be used for male sex determination. Definite signals following
PCR amplification were obtained in all cases indicated that single blastomere cell from preimplantation of bovine
embryos was sufficient for male sex determination. Male sex determination of the embryos was developed
further in both in vitro and in vivo successfully. |
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