IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE

The aims of this study was to identify Aeromonas salmonicida as the cause of Furunculosis disease in gouramy (Osphronemus gouramy) using conventional and polymerase chain reaction technique. Ten gills and skin swab samples were obtained from suspected Furunculosis gouramy. The samples were isolat...

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Main Author: RUTH TYAS ADVENDTINE, 061211133080
Format: Theses and Dissertations NonPeerReviewed
Language:English
Indonesian
Published: 2016
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Online Access:http://repository.unair.ac.id/60425/1/ABSTRACT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf
http://repository.unair.ac.id/60425/2/FULLTEXT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf
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Institution: Universitas Airlangga
Language: English
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spelling id-langga.604252017-08-14T18:35:28Z http://repository.unair.ac.id/60425/ IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE RUTH TYAS ADVENDTINE, 061211133080 QP501-801 Animal biochemistry QR180 Immunology The aims of this study was to identify Aeromonas salmonicida as the cause of Furunculosis disease in gouramy (Osphronemus gouramy) using conventional and polymerase chain reaction technique. Ten gills and skin swab samples were obtained from suspected Furunculosis gouramy. The samples were isolated on Trypticase Soy Agar and MacConkey Agar, than the colonies from that medium followed by identification tests using Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), Urea Agar, and Sugar Fermentation Test. Sugar fermentation tests that were used in this study was consists of Glucose, Lactose, Sucrose, Maltose, and Manitol. From these test, 3 out of 10 samples were identified as Aeromonas salmonicida. Based on the biochemical tests result that the bacteria could be determined as Aeromonas salmonicida, the PCR test was done for the confirmation. Ferric siderophore receptor gene were used as specific gene to identify Aeromonas salmonicida. The confirmatory test shows band at 389 bp. 2016 Thesis NonPeerReviewed text en http://repository.unair.ac.id/60425/1/ABSTRACT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf text id http://repository.unair.ac.id/60425/2/FULLTEXT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf RUTH TYAS ADVENDTINE, 061211133080 (2016) IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE. Skripsi thesis, Universitas Airlangga. http://lib.unair.ac.id
institution Universitas Airlangga
building Universitas Airlangga Library
country Indonesia
collection UNAIR Repository
language English
Indonesian
topic QP501-801 Animal biochemistry
QR180 Immunology
spellingShingle QP501-801 Animal biochemistry
QR180 Immunology
RUTH TYAS ADVENDTINE, 061211133080
IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE
description The aims of this study was to identify Aeromonas salmonicida as the cause of Furunculosis disease in gouramy (Osphronemus gouramy) using conventional and polymerase chain reaction technique. Ten gills and skin swab samples were obtained from suspected Furunculosis gouramy. The samples were isolated on Trypticase Soy Agar and MacConkey Agar, than the colonies from that medium followed by identification tests using Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), Urea Agar, and Sugar Fermentation Test. Sugar fermentation tests that were used in this study was consists of Glucose, Lactose, Sucrose, Maltose, and Manitol. From these test, 3 out of 10 samples were identified as Aeromonas salmonicida. Based on the biochemical tests result that the bacteria could be determined as Aeromonas salmonicida, the PCR test was done for the confirmation. Ferric siderophore receptor gene were used as specific gene to identify Aeromonas salmonicida. The confirmatory test shows band at 389 bp.
format Theses and Dissertations
NonPeerReviewed
author RUTH TYAS ADVENDTINE, 061211133080
author_facet RUTH TYAS ADVENDTINE, 061211133080
author_sort RUTH TYAS ADVENDTINE, 061211133080
title IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE
title_short IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE
title_full IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE
title_fullStr IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE
title_full_unstemmed IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE
title_sort identification of aeromonas salmonicida as the cause of furunculosis disease on gouramy (osphronemus gouramy) using conventional and polymerase chain reaction technique
publishDate 2016
url http://repository.unair.ac.id/60425/1/ABSTRACT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf
http://repository.unair.ac.id/60425/2/FULLTEXT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf
http://repository.unair.ac.id/60425/
http://lib.unair.ac.id
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