IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE
The aims of this study was to identify Aeromonas salmonicida as the cause of Furunculosis disease in gouramy (Osphronemus gouramy) using conventional and polymerase chain reaction technique. Ten gills and skin swab samples were obtained from suspected Furunculosis gouramy. The samples were isolat...
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id-langga.604252017-08-14T18:35:28Z http://repository.unair.ac.id/60425/ IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE RUTH TYAS ADVENDTINE, 061211133080 QP501-801 Animal biochemistry QR180 Immunology The aims of this study was to identify Aeromonas salmonicida as the cause of Furunculosis disease in gouramy (Osphronemus gouramy) using conventional and polymerase chain reaction technique. Ten gills and skin swab samples were obtained from suspected Furunculosis gouramy. The samples were isolated on Trypticase Soy Agar and MacConkey Agar, than the colonies from that medium followed by identification tests using Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), Urea Agar, and Sugar Fermentation Test. Sugar fermentation tests that were used in this study was consists of Glucose, Lactose, Sucrose, Maltose, and Manitol. From these test, 3 out of 10 samples were identified as Aeromonas salmonicida. Based on the biochemical tests result that the bacteria could be determined as Aeromonas salmonicida, the PCR test was done for the confirmation. Ferric siderophore receptor gene were used as specific gene to identify Aeromonas salmonicida. The confirmatory test shows band at 389 bp. 2016 Thesis NonPeerReviewed text en http://repository.unair.ac.id/60425/1/ABSTRACT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf text id http://repository.unair.ac.id/60425/2/FULLTEXT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf RUTH TYAS ADVENDTINE, 061211133080 (2016) IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE. Skripsi thesis, Universitas Airlangga. http://lib.unair.ac.id |
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QP501-801 Animal biochemistry QR180 Immunology RUTH TYAS ADVENDTINE, 061211133080 IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy) USING CONVENTIONAL AND POLYMERASE CHAIN REACTION TECHNIQUE |
description |
The aims of this study was to identify Aeromonas salmonicida as the cause
of Furunculosis disease in gouramy (Osphronemus gouramy) using conventional
and polymerase chain reaction technique. Ten gills and skin swab samples were
obtained from suspected Furunculosis gouramy. The samples were isolated on
Trypticase Soy Agar and MacConkey Agar, than the colonies from that medium
followed by identification tests using Triple Sugar Iron Agar (TSIA), Sulfide
Indole Motility (SIM), Simmons Citrate Agar (SCA), Urea Agar, and Sugar
Fermentation Test. Sugar fermentation tests that were used in this study was
consists of Glucose, Lactose, Sucrose, Maltose, and Manitol. From these test, 3
out of 10 samples were identified as Aeromonas salmonicida. Based on the
biochemical tests result that the bacteria could be determined as Aeromonas
salmonicida, the PCR test was done for the confirmation. Ferric siderophore
receptor gene were used as specific gene to identify Aeromonas salmonicida. The
confirmatory test shows band at 389 bp. |
format |
Theses and Dissertations NonPeerReviewed |
author |
RUTH TYAS ADVENDTINE, 061211133080 |
author_facet |
RUTH TYAS ADVENDTINE, 061211133080 |
author_sort |
RUTH TYAS ADVENDTINE, 061211133080 |
title |
IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF
FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy)
USING CONVENTIONAL AND POLYMERASE
CHAIN REACTION TECHNIQUE |
title_short |
IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF
FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy)
USING CONVENTIONAL AND POLYMERASE
CHAIN REACTION TECHNIQUE |
title_full |
IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF
FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy)
USING CONVENTIONAL AND POLYMERASE
CHAIN REACTION TECHNIQUE |
title_fullStr |
IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF
FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy)
USING CONVENTIONAL AND POLYMERASE
CHAIN REACTION TECHNIQUE |
title_full_unstemmed |
IDENTIFICATION OF Aeromonas salmonicida AS THE CAUSE OF
FURUNCULOSIS DISEASE ON GOURAMY (Osphronemus gouramy)
USING CONVENTIONAL AND POLYMERASE
CHAIN REACTION TECHNIQUE |
title_sort |
identification of aeromonas salmonicida as the cause of
furunculosis disease on gouramy (osphronemus gouramy)
using conventional and polymerase
chain reaction technique |
publishDate |
2016 |
url |
http://repository.unair.ac.id/60425/1/ABSTRACT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf http://repository.unair.ac.id/60425/2/FULLTEXT%20KKC%20KK%20KH.%2074.17%20Adv%20i.pdf http://repository.unair.ac.id/60425/ http://lib.unair.ac.id |
_version_ |
1681148117187035136 |