ISOLASI DAN IDENTIFIKASI VIRUS NEWCASTLE DISEASE (ND) DARI ORGAN PENCERNAAN AYAM BROILER YANG TERINDIKASI SAKIT DI PASAR TRADISIONAL SURABAYA
The aim of this research to confirm the presence of Newcastle Disease virus on broiler chicken in the traditional market environment via virus isolation and identification. This research is using 30 post-mortem colon samples of broiler chickens collected from traditional markets in Surabaya. Susp...
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| Main Author: | |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/61251/1/ABSTRACT%20KKC%20KK%20KH.%20225.17%20War%20i.pdf http://repository.unair.ac.id/61251/2/FULLTEXT%20KKC%20KK%20KH.%20225.17%20War%20i.pdf http://repository.unair.ac.id/61251/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | English Indonesian |
| Summary: | The aim of this research to confirm the presence of Newcastle Disease
virus on broiler chicken in the traditional market environment via virus isolation
and identification. This research is using 30 post-mortem colon samples of broiler
chickens collected from traditional markets in Surabaya. Suspected materials
inoculated into allantoic sac in specific antigen negative of 10 days embryonating
chicken’s egg. It had been conducted in Virology and Immunology Laboratory,
Department of Microbiology, Faculty of Veterinary Medicine, University of
Airlangga since May 2017 until June 2017. Methods that used are
hemaglutination (HA) test and hemaglutination inhibition (HI) test. The growth of
the virus determined by the ability to agglutinate chicken red blood cells or
hemaglutination test. Positive hemaglutination performed by hemaglutination
inhibition (HI) test using specific antibody against ND virus. The results showed
that from the 30 samples which isolated from colon of post-mortem poultry
showing symptoms of Newcastle disease virus infection, a total of 1 sample from
30 samples (0,03%) is positive tested for Newcastle disease virus infection. |
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