Potensi Mutan Δf 508-T Gen Pengkode Cystic Fibrosis Transmembrane Conductance Regulator (Cftr) Sebagai Prototype Pada Penyakit Congenital Bilateral Absence Of Vas Deferens (Cbavd) Di Indonesia

• Main Author: Maslichah Mafruchati
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian
• Published: 2015
• Subjects: R735-854 Medical education. Medical schools. Research
• Online Access: http://repository.unair.ac.id/63550/1/1.%20HALAMAN%20JUDUL.pdf; http://repository.unair.ac.id/63550/2/2.%20ABSTRAK.pdf; http://repository.unair.ac.id/63550/3/3.%20BAB%201.pdf; http://repository.unair.ac.id/63550/4/4.%20BAB%202.pdf; http://repository.unair.ac.id/63550/5/5.%20BAB%203.pdf; http://repository.unair.ac.id/63550/6/6.%20BAB%204.pdf; http://repository.unair.ac.id/63550/7/7.%20BAB%205.pdf; http://repository.unair.ac.id/63550/8/8.%20BAB%206.pdf; http://repository.unair.ac.id/63550/9/9.%20BAB%207.pdf; http://repository.unair.ac.id/63550/10/10.%20DAFTAR%20PUSTAKA.pdf; http://repository.unair.ac.id/63550/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

CBAVD is congenital defect, data concerning the report of CBAVD case have not been reporting in Indonesia. The aim of this research to determine potency mutan ΔF 508-T gene CFTR could be used as prototype for therapy congenital defect CBAVD in Indonesia. Blood sample from Azoospermi man was collected and measured by R Neacy-QIAGENKit to compare their quality. Three different primers were used in this research. The result of SDS-PAGE method was positive to find molecular weight CFTR protein in CBAVD patient. The result of research showed that man with CBAVD have level base pair on 400bp in CBAVD patient in Indonesia, detection of Adenovirus was negative by PCR, this support that was truly congenital disease, The result of SDS_PAGE to measure molecular weight CFTR protein were 190-250kDA, 170-180k Da, 140-160kDA and 120kDa and the score of Immunoglobuline G was 15579 mg/dl measured by Cobas C6000 Immunoturbidimetri. The level base pair on 400 bp of DNA mutant ΔF 508-T gene Code CFTR will guide strategies for developing new therapeutics and alternative theraphy toward defect congenital CBAVD in Indonesia