MEKANISME REGENERASI DEFEK KARTILAGO MANDIBULA PADA IMPLANTASI HUMAN UMBILICAL CORD MESENCHYMAL STEM CELL DENGAN SCAFFOLD PLATELET RICH FIBRIN
Mandibular cartilage is a fibrocartilage tissue, as well as other articular cartilage have a limited regenerative capacity. Various technique that had been developed previously did not provide satisfying long term result. The advantage of human umbilical cord mesenchymal stem cell such as ability...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/64289/1/Dis.K.02.17%20.%20Sum.m%20-%20ABSTRAK.pdf http://repository.unair.ac.id/64289/2/Dis.K.02.17%20.%20Sum.m%20-%20SEC.pdf http://repository.unair.ac.id/64289/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Mandibular cartilage is a fibrocartilage tissue, as well as other articular cartilage
have a limited regenerative capacity. Various technique that had been developed
previously did not provide satisfying long term result. The advantage of human
umbilical cord mesenchymal stem cell such as ability to differetiated into
osteogenic, adipogenic, and chondrogenic lineage, made hUCMSC a choice
treatment in cartilage regeneration, however its regenerative mechanisms has not
yet been understood. The aim of this study was to describe the mechanism of
mandibular cartilage regeneration with implantation of hUCMSC over PRF
scaffold. Twenty Rattus novergicus were divided into 4 groups: 1 control group
and 3 experimental groups. First experimental group was implantation of
hUCMSC on mandibular cartilage defect, second experimental group was
implantation of PRF, and third experimental group was implantation of hUCMSC
over PRF scaffold. After 6 weeks of observation, 20 experimental animal were
sacrificed for histopatology and immunohistochemisty staining. Expression of
FGF 18, Sox 9, Ki67, chondrocyte counts, type II collagen, aggrecan, and
cartilage thickness were analyzed. Expression of FGF 18, Sox 9, Ki67,
chondrocyte counts, type II collagen, aggrecan, and cartilage thickness were
significantly higher ini hUCMSC over PRF scaffold group showed that PRF as
scaffold support chondrocyte differentiation of hUCMSC and the differentiated
chondrocyte were able to form cartilage matrix in mandibular cartilage
regeneration. Different pathway involved in mandibular cartilage thickness
increase in each group. Regeneration of mandibular cartilage with implantation of
hUCMSC over PRF scaffold establihed through increasing expresssion of FGF 18
that upregulated Sox 9 expression, enhanced chondocyte differentiation of
hUCMSC, thus the differentiated chondrocyte formed extracellular matrix such as
type II collagen and aggrecan that form mandibular cartilage thickness. |
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