EKSPRESI ANTIBODI PROTEIN BM 41.5 kDa PADA SEL LIMFOSIT MENCIT BALB/C PASCA INDUKSI PROTEIN BM 41.5 kDa SPERMATOZOA MANUSIA UNTUK PRODUKSI ANTIBODI MONOKLONAL (Pengembangan Produk Imunodiagnostik Kit Detekesi Fertilitas Pria)
This male fertility detection test uses the immunodiagnostic principle in which defined as a diagnostic test using markers based on antigen-antibody reactions. Monoclonal antibodies are homogeneous, have high specificity and can be produced continuously. According to the developmet of ethanolic...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/65193/1/FF.FT.37.17%20.%20Zul.e%20-%20ABSTRAK.pdf http://repository.unair.ac.id/65193/2/FF.FT.37.17%20.%20Zul.e%20-%20SEC.pdf http://repository.unair.ac.id/65193/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | This male fertility detection test uses the immunodiagnostic
principle in which defined as a diagnostic test using markers based on
antigen-antibody reactions. Monoclonal antibodies are homogeneous,
have high specificity and can be produced continuously. According to the
developmet of ethanolic extract of Justicia gendarussa Burm.f as a male
oral contraceptive drug, it is known that human spermatozoa protein with
molecular weight of 38 kDa and 41.5 kDa play an important role in sperm
fertility. Human spermatozoa protein with molecular weight of 41.5 kDa
has been known to have a function as glycoprotein and hyaluronidase
enzyme. The objective of this study was to discover the immunogenic
properties of 41.5 kDa protein isolated from spermatozoa membrane
using SDS-PAGE method, confirmed by performing specificity and
sensitivity test of 41.5 kDa protein antibody using ELISA and western
blot methods and to discover the ability of mice’s lymphocyte cell to
express 41.5 kDa protein antibody before performing the cell fusion stage
with myeloma cell using immunocytochemistry method. The results
showed that the highest antibody titer was on the fourth bleeding with
absorbance value of 0.522 and 0.523. It indicated that the induction result
of human spermatozoa protein with molecular weight of 41,5 kDa had
immunogenic properties and specific to 41,5 kDa protein and mice’s
lymphocyte cell had the ability to express antibody showed by the highest
expression on 4th week with immunoration of 94, 900 ± 3,174.
Furthermore, lymphocyte can be used in the stage of monoclonal
antibody formation and thus, further research on the formation of
monoclonal antibody is needed to develop immunodiagnostic detector for
male fertility. |
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