UJI TOKSISITAS EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi L) TERSTANDAR KUERSETIN PADA MENCIT PUTIH (Mus musculus)
Belimbing wuluh leaves (Averrhoa bilimbi L) contains alkaloid compounds, carbohydrates, flavonoids, glycosides, phenols, steroids and tannins. Flavonoids are a group of organic compounds that are spread evenly throughout the plant part of one of the components is quercetin compound. The querceti...
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | Indonesian Indonesian |
Published: |
2017
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Subjects: | |
Online Access: | http://repository.unair.ac.id/65280/1/FF.KF.40.17%20.%20Wul.u%20-%20ABSTRAK.pdf http://repository.unair.ac.id/65280/2/FF.KF.40.17%20.%20Wul.u%20-%20SEC.pdf http://repository.unair.ac.id/65280/ http://lib.unair.ac.id |
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Institution: | Universitas Airlangga |
Language: | Indonesian Indonesian |
Summary: | Belimbing wuluh leaves (Averrhoa bilimbi L) contains
alkaloid compounds, carbohydrates, flavonoids, glycosides, phenols,
steroids and tannins. Flavonoids are a group of organic compounds
that are spread evenly throughout the plant part of one of the
components is quercetin compound. The quercetin contained in the
belimbing wuluh leaf has several pharmacological effects, and to
support its use in the treatment it is important to evaluate the safety
of quercetin through an acute toxicity test. The aim of this study is to
determine the toxicity of belimimbing wuluh leaf extract (Averrhoa
bilimbi L) standardized quercetin in white mice (Mus musculus).
The method used in determining the level of quercetin in
leaf extract using UV Spectrophotometry. The toxicity test method
uses conventional methods which use 4 different doses. The given
dose of standarized extract includes 200 mg / kgBW; 600 mg /
kgBW; 1800 mg / kgBW; 5400 mg / kgBW The experimental animal
used is white mouse (Mus musculus) and the number mice used is six
per group.
In this study, the level of quercetin in the extract of
leaves of belimbing wuluh was 5,42 ± 1,77 (% ; w/w). Toxicity test
results showed no mortality in experimental animals in the
observation 24 hours and 48 hours. Mice mortality data were
analyzed using three methods: Wheil method, probit method in IBM
SPSS Statistic 23 and regression method. The analysis results show
that LD50 value can not be determined because there is no animal
mortality. Additional observations were performed on weight
changes indicated by weight gain after administration of the test
preparation. Other observations were made at locomotoric activity
after 24 hours administration of the test preparation. Results showed
no change or decrease in animal experiments try. The above data
concluded that the LD50 value of more than 5400 mg / KgBB showed
the extract of leaves of wuluh belimbing wuluh practically not toxic. |
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