UJI AKTIVITAS ANTIMIKROBA EKSTRAK ETANOL BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI Salmonella typhi SECARA in vitro
Morinda Citrifolia L. ethanol extract can be used as traditional medicine is to cure typhoid fever. While lately, many resistance cases of this Salmonella typhi bacteria against many kinds of antibiotic that usually used such as chloramphenicol, amphisillin, and tetracycline. Therefore, we decide...
Saved in:
Main Author: | |
---|---|
Format: | Theses and Dissertations NonPeerReviewed |
Language: | Indonesian Indonesian |
Published: |
2017
|
Subjects: | |
Online Access: | http://repository.unair.ac.id/66327/1/FK%20PD%2096-17%20Ath%20u%20Abstrak.pdf http://repository.unair.ac.id/66327/2/FK%20PD%2096-17%20Ath%20u%20Sec.pdf http://repository.unair.ac.id/66327/ http://lib.unair.ac.id |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universitas Airlangga |
Language: | Indonesian Indonesian |
Summary: | Morinda Citrifolia L. ethanol extract can be used as traditional medicine is to cure
typhoid fever. While lately, many resistance cases of this Salmonella typhi bacteria
against many kinds of antibiotic that usually used such as chloramphenicol, amphisillin,
and tetracycline. Therefore, we decide to conduct this research. The objective of this
research to find out the potential effect of antibacterial Morinda Citrifolia L. ethanol
extract against Salmonella typhi bacteria.
This research was a microbiology laboratory experimental which is conducted in
in vitro way, by using dilution to determine the MIC and MBC, continued by streaking
on NAP. The extract concentration used in this research were 2gr/ml, 1gr/ml, 0,5gr/ml,
0,25gr/ml, 0,125gr/ml, 0,0625gr/ml, 0,0312gr/ml, and 0,0156gr/ml. This experiment is
replicated three times. MIC was observed by the significant alterations of solution’s
turbidity after incubation at 37ºC for 24 hours. All those solutions then cultured on NAP
at 37ºC for 24 hours and observed visually the growth of bacterial colonies.
MIC on this research was 0,0625gr/mL meanwhile the MBC was 0,25gr/ml.
In conclusion, Morinda Citrifolia L. ethanol extract has antibacterial effect against
Salmonella typhi with MIC of 0,0625gr/ml and MBC of 0,25gr/ml. |
---|