PENGEMBANGAN DAN VALIDASI METODE KCKT PADA PENENTUAN KADAR MORAKHALKON A SEBAGAI SENYAWA MARKER DALAM EKSTRAK ETANOL 80% KULIT BATANG Artocarpus champeden SPRENG
Artocarpus champeden SPRENG., commonly known as “cempedak” has been traditionally used for malarial remedies. In order to develop A. champeden as antimalarial phytopharmaceutical product, marker approach was choosen for quality control purpose of the extract as raw material. Morachalchone A is th...
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| 格式: | Theses and Dissertations NonPeerReviewed |
| 語言: | English English |
| 出版: |
2017
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| 主題: | |
| 在線閱讀: | http://repository.unair.ac.id/66430/1/abstrak.pdf http://repository.unair.ac.id/66430/2/Tesis%20Evi%20Kurniawati.compressed.pdf http://repository.unair.ac.id/66430/ |
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| 機構: | Universitas Airlangga |
| 語言: | English English |
| 總結: | Artocarpus champeden SPRENG., commonly known as “cempedak” has
been traditionally used for malarial remedies. In order to develop A. champeden
as antimalarial phytopharmaceutical product, marker approach was choosen for
quality control purpose of the extract as raw material. Morachalchone A is the
active marker of ethanol extract Artocarpus champeden. To ensure the quality,
efficacy and safety of the phytopharmaceutical products, a simple and selective
analytical method becomes important for the determination of morachalchone A
in ethanol extract 80% sample. The purpose of this study was to develop and
validate a simple and selective High Performance Liquid Chromatography
(HPLC) for determination of morachalchone A in ethanol extract 80%
sample.This method was performed using a RP-18 Column 5μm (250 x 4.6 mm
i.d) as stationary phase, isocratic mobile phase of acetonitrile : water (50:50 v/v)
with flow rate 1.0 ml/minute, injection volume 20 μl and analytical wavelength at
368 nm. The results showed that method was selective to separate morachalcone
A from other component with good resolution. Retention time morachalcone A
was 21.520 minute. The data for calibration plots showed good linear relationship
with r = 0.999 in the concentration range 1.93 - 7.71 ppm. The limit of detection
and quantification were found 0.26 ppm and 0.86 ppm, respectively. The mean of
% recovery addition standard method in 80%, 100% and 120% was found
102,72% ±2,47; 97,37% ±2,18 and 96,66% ±0,80. The relative standard deviation for
repeatability and intermediate precision was 4.47% and 3.19% respectively. The
marker content in ethanol extract of Artocarpus champeden SPRENG stembark
was shown as mean ± SD (RSD) of 0.0151% ± 0.0002 (1.83%) |
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