IN VITRO STUDY ON ANTI – AMOEBIC ACTIVITY OF MANGOSTEEN (Garcinia mangostana L.) PERICARP EXTRACT AGAINST Entamoeba histolytica
Background: Entamoeba histolytica is the responsible protozoan that acts as causative agent of human amoebiasis. It is the second leading cause of death worldwide after malaria. Approximately, 50 million people worldwide suffer from invasive amoebic infection annually. Anti-bacterial properties o...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/66496/1/FK.PD.194.17%20.%20Har.i%20-%20ABSTRAK.pdf http://repository.unair.ac.id/66496/2/FK.PD.194.17%20.%20Har.i%20-%20SEC.pdf http://repository.unair.ac.id/66496/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | English English |
| Summary: | Background: Entamoeba histolytica is the responsible protozoan that acts as
causative agent of human amoebiasis. It is the second leading cause of death
worldwide after malaria. Approximately, 50 million people worldwide suffer from
invasive amoebic infection annually. Anti-bacterial properties of mangosteen
(Garcinia mangostana L.) pericarp have been widely studied. However, the antiamoebic
activity of mangosteen pericarp against Entamoeba histolytica is still not
known. This preliminary study was conducted to evaluate the in vitro activity of
mangosteen pericarp against Entamoeba histolytica.
Methods: This research is an experimental study. Anti-amoebic activity of
mangosteen pericarp was assessed using broth dilution method. Different
concentration of mangosteen pericarp (400μg/ml; 200μg/ml; 100μg/ml; 80μg/ml;
40μg/ml; 20μg/ml; 10μg/ml; 1μg/ml) were tested against HM-1:IMSS stain
E.histolytica in 96-well flat-bottom microtiter plate under anaerobic condition. After
24 hours of incubation, the growth of trophozoites in each well was evaluated using
spectrophotometer. The percentage viability is calculated. Minimum inhibitory
concentration was defined as the lowest concentration of mangosteen (Garcinia
mangostana L.) pericarp extract inhibits the growth of Entamoeba histolytica after 24
hours of incubation. This experiment was repeated three times and triplicate
replication.
Result: Anti-amoebic activity and minimum inhibitory concentration (MIC) of
mangosteen (Garcinia mangostana L.) pericarp extract against Entamoeba histolytica
cannot be determined in this study because the unstable condition of cells and
environmental laboratory factors that cannot be avoided.
Conclusion: This preliminary study shows the anti-amoebic activity and minimum
inhibitory concentration (MIC) of mangosteen (Garcinia mangostana L.) pericarp
extract against Entamoeba histolytica cannot be determined. Further research is
needed, especially in vivo experiment. |
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