IDENTIFIKASI MDR (Multi Drugs Resistance ) MELALUI GEN Int1 (Integron Class 1) Citrobacter freundii PADA TELUR AYAM DENGAN METODE PCR (Polymerase Chain Reaction)
Chicken eggs are a rich source of protein; therefore, eggs are easily contaminated by bacteria, especially bacteria from the Enterobacteriaceae family. C. freundii is a member of the Enterobacteriaceae family that can cause gastrointestinal infections in humans through the consumption of contamin...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/66718/1/TPKMV.08.17%20.%20Put.i%20-%20ABSTRAK.pdf http://repository.unair.ac.id/66718/2/TPKMV.08.17%20.%20Put.i%20-%20SEC.pdf http://repository.unair.ac.id/66718/ http://lib.uniar.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Chicken eggs are a rich source of protein; therefore, eggs are easily
contaminated by bacteria, especially bacteria from the Enterobacteriaceae family. C.
freundii is a member of the Enterobacteriaceae family that can cause gastrointestinal
infections in humans through the consumption of contaminated food. Besides being
found on food, C. freundii is also often isolated from soil, water, feces, waste
materials, and intestines. C. freundii plays an active role in carrying a resistance gene
in the gastrointestinal tract of both humans and animals and can contaminate animalbased
food products through feces. This study aimed to discover whether the MDRcoding
gene, Int1, could be found in C. freundii isolates. The isolation and
identification of C. freundii in this study used LB as the pre-enrichment medium,
TTB as the enrichment medium, and the medium SSA as a selective medium,
followed by Gram staining of colonies, biochemical tests (TSIA, Urea, SCA, SIM,
MR-VP), carbohydrate fermentation tests (lactose, sucrose, glucose, mannitol, and
maltose) and a validation test using the Vitek 2 machine. Tests for resistance to a
number of antibiotics using the antibiotic disc diffusion method were conducted for
streptomycin, gentamycin, chloramphenicol, erythromycin, tetracycline,
oxytetracycline, doxycycline, amoxicillin, penicillin, oxacillin, cloxacillin,
trimethoprim, and ciprofloxacin. The results study demonstrated that C. freundii was
100% resistant to erythromycin, amoxicillin, penicillin, oxacillin, and cloxacillin, and
33.3% resistant to ampicillin. The Polymerase Chain Reaction (PCR) analysis of C.
freundii revealed DNA strand fragments at a fragment length of 346 bp. The PCR
product sequencing analysis was conducted to see the homology of the DNA. The
result of the homology was 91% by using the BLAST system. |
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