UJI KEPEKAAN ANTIBIOTIK DAN IDENTIFIKASI GEN PENYANDI ENTEROTOKSIN C PADA Staphylococcus aureus DARI SUSU SAPI DI BEBERAPA PETERNAKAN JAWA TIMUR
The aim of this research was to know sensitivity antibiotics and identification encoding gene enterotoxin C in S. aureus from milk in several farms of East Java. Isolated S. aureus that was resistant to MRSA from milk and acknowledged the different sensitivity of S. aureus to some antibiotics as...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/66728/1/TPKMV.13.17%20.%20Wan.u%20-%20ABSTRAK.pdf http://repository.unair.ac.id/66728/2/TPKMV.13.17%20.%20Wan.u%20-%20SEC.pdf http://repository.unair.ac.id/66728/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | The aim of this research was to know sensitivity antibiotics and
identification encoding gene enterotoxin C in S. aureus from milk in several
farms of East Java. Isolated S. aureus that was resistant to MRSA from milk and
acknowledged the different sensitivity of S. aureus to some antibiotics as well as
amplified the encoding genes of enterotoxin C in MRSA and MSSA isolates. This
study was conducted in vitro by streak method for S. aureus isolate, susceptibility
test antibiotics with Kirby-Bauer Agar diffusion method with Mc Farland 0.5
standard, MRSA detection test using Cefoxitin disk diffusion on Mueller Hinton
Agar and PCR technique for encoding gene of enterotoxin C in S. aureus and
MRSA. As many 148 milk samples, 24 positive isolates of S. aureus were
obtained. The colonies can be fermented mannitol, shape like a coccus, Gram
positive, produce katalase, and koagulase. Sensitivity to some antibiotics that
show erythromycin is an antibiotic that has the highest level of sensitivity reaches
100%. MRSA detection test contained one positive isolate, PCR test showed two
isolates MSSA had enterotoksin C gene with molecular weight of 257 bp. Based
on the result of this study it is advisable to conduct other antibiotic sensitivity tests
to determine the sensitivity of antibiotics agains S. aureus, and should be aware
milk can also be reservoir MRSA to humans, also need to be futher research on
the presence of other types enterotoxin encoding gene derived from milk. |
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