EFEKTIFITAS EKSTRAK PROPOLIS TERHADAP EXTRACELLULAR POLYMERIC SUBSTANCE (EPS) BIOFILM BAKTERI Enterococcus Faecalis

• Main Author: DHEA ADITTYA, drg, 021418036309
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian
• Published: 2017
• Subjects: RK Dentistry
• Online Access: http://repository.unair.ac.id/66905/1/PPDGS.KON.16.17%20.%20Adi.e%20-%20ABSTRAK.pdf; http://repository.unair.ac.id/66905/2/PPDGS.KON.16.17%20.%20Adi.e%20-%20SEC.pdf; http://repository.unair.ac.id/66905/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

Backgorund: The resistance of Enterococcus faecalis bacteria in root canals is because it can form a biofilm. Biofilms are communities of microorganisms in a complex and dynamic structure attached to a surface, embedded in Extracellular Polymeric Substance (EPS). Propolis extract contains of flavonoids that can inhibit biofilm growth. Objective: To analyze the effectiveness of propolis extracts against Enterococcus faecalis biofilm EPS in vitro. Materials and Methods: Enterococcus faecalis biofilm grown on media Tryptic Soy Broth (TSB), dextran conjugate alexa fluor 647 reagent was added, then incubated 24 hours. Enterococcus faecalis biofilm were divided into 4 groups: 3 groups treated by soaking propolis extracts 24 hours with each concentration of 02, 0.8% and 1.2%; 1 control group without extract of propolis. Biofilm samples examined using Confocal Laser Scanning Microscope (CLSM). Yield data was then analyzed using One Way ANOVA and Tukey HSD Test (p <0.05). Results: The treatment group showed a decrease in the average volume of biofilm EPS than the control group. Conclusion: propolis extracts with concentration of 0.2% 0.8% and 1.2%, effectively reducing the Enterococcus faecalis biofilm EPS.