EFEKTIVITAS EKSTRAK KULIT MANGGIS (GARCINIA MANGOSTANA L) TERHADAP LIPOTEICHOIC ACID (LTA) ENTEROCOCCUS FAECALIS MELALUI EKSPRESI TLR-2 DAN TLR-4 PADA HUMAN PERIODONTAL LIGAMENT FIBROBLASTS (HPDLFS)
Background : Enterococcus faecalis is a microorganism commonly detected in persistent endodontic infections. Its prevalence in such infections ranges from 24% to 77%. Lipoteichoic acid (LTA) is a major virulence factor of Enterococcus faecalis acts as an adhesion molecule binds to host cells via...
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| Main Author: | |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/66908/1/PPDGS.KON.17.17%20.%20Jes.e%20-%20ABSTRAK.pdf http://repository.unair.ac.id/66908/2/PPDGS.KON.17.17%20.%20Jes.e%20-%20SEC.pdf http://repository.unair.ac.id/66908/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Background : Enterococcus faecalis is a microorganism commonly detected in persistent
endodontic infections. Its prevalence in such infections ranges from 24% to 77%.
Lipoteichoic acid (LTA) is a major virulence factor of Enterococcus faecalis acts as an
adhesion molecule binds to host cells via its lipid moiety and promote bacterial colonization
and invasion. LTA recognized by TLR2 and TLR4 that are expressed on periodontal ligament
cells. Binding of TLR and LTA leads to recruitment of inflammatory cells and also cytokines
and subsequent osteoclast activation which leads to destruction of periodontal and periapical
lessions. Root canal irrigation materials that are widely used nowadays is NaOCl, but this
material has many shortcomings. The pericarp of Garcinia Mangostana that can be an
alternative material which could inhibit and bactericidal function to Enterococcus faecalis.
This experimental research was done using human periodontal ligament fibroblasts (HPdLFs)
to examine if Garcinia Mangostana extract can reduce the amount of LTA by the expression
of TLR-2 and TLR-4 Purpose : The aim of this study was to know the effectiveness of
Garcinia Mangostana extract to LTA by the expression of TLR-2 and TLR-4 in human
periodontal ligament fibroblasts. Method : This research was a laboratory experimental. Two
groups of HPdLFs culture were induced with Garcinia Mangostana extract within
concentration 5 μg/ml, 6 μg/ml, 7 μg/ml, 8 μg/ml, 9 μg/ml, 10 μg/ml. And each group then
induced with LTA 15 μg and 30 μg. The number of sample of each group is 3 (n=3). After
that, observed the result of the amount of TLR-2 and TLR-4 by using immunocytochemistry.
Results : There were significant differences between each group (p<0,05). Conclusion :
Garcinia Mangostana extract lower the LTA by the expression of TLR-2 and TLR-4 in
human periodontal ligament and the most effective concentration is 10 μg/ml. |
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