POLA PASANGAN KUMAN PEMBENTUK BIOFIL DAN UJI SENSITIVITAS ANTIBIOTIK PADA PASIEN DI RUANG RAWAT INAP INTENSIVE RSUD DR. SOETOMO SURABAYA
Biofilm-associated microorganisms can cause disease by attachment of individual or group cells on the surface of medical device and have chance to grow as organisms that are resistant to antibiotics. These microorganisms can be prokaryote or eukaryote organisms that can exist in one of two forms,...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/67107/1/TMBK%2001-17%20Fur%20p%20abstrak.pdf http://repository.unair.ac.id/67107/2/TMBK%2001-17%20Fur%20p%20fulltext.pdf http://repository.unair.ac.id/67107/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Biofilm-associated microorganisms can cause disease by attachment of
individual or group cells on the surface of medical device and have chance to grow
as organisms that are resistant to antibiotics. These microorganisms can be
prokaryote or eukaryote organisms that can exist in one of two forms, i. e., sessile
or planktonic. Treatment of device-associated infection with systemic antimicrobial
agent usually ineffective. Biofilm-forming organisms on medical devices can be
Gram-positive bacteria, Gram-negative bacteria, or yeasts. By knowing the
presence of biofilm on medical devices, one can avoid quorum sensing which leads
to treatment failure due to antibiotic resistance.
Purpose: To pattern the biofilm-forming organisms and their antimicrobial
susceptibility test from medical devices on patients so that therapeutic management
of patients with biofilms can be more accurate by giving antibiotic according to the
species identified and the antimicrobial susceptibility test.
Method: From 86 specimens that were analyzed, only 36 specimens that
showed organism growth and ability to form biofilm in Microtiter Plate Assay.
From 36 isolates that were analyzed for the ability to form biofilm, only 31 isolates
that can be planktonic and sessile, which then identified and tested for antimicrobial
susceptibility because the density produced were more or equal to 0,4 ( cutt off ≥
0,4 ).
Result: From the organisms obtained from devices by Microtiter Plate
Assay, the distribution and antimicrobial susceptibility pattern were made based on
planktonic and sessile form of biofilm. Planktonic Escherichia coli from urinary
and intravenous catheters matched 100% with the sessile form on the devices, as
well as Klebsiella pneumoniae and Pseudomonas aeruginosa. Gram-positive cocci
found was Staphylococcus aureus. Planktonic Staphylococcus aureus from urinary
and intravenous catheters matched 100% with the sessile form on the devices. The
emphasize was that sterile form on planktonic biofilm does not always means that
the sessile form was also sterile.
Conclusion: Microtiter Plate assay method can be used to pattern pattern
the biofilm-forming organisms and their antimicrobial susceptibility test based on
the planktonic and sessile forms of biofilm, therefore helping clinicians in deciding
the right antibiotic treatment. |
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