The differences of effectiveness of β -1,3-glukanase Vigna unguiculata and papain Carica papaya enzymes in hydrolysis of denture plaque
Background: Accumulation of denture plaque can lead to pathological changes in oral mucosa, such as denture stomatitis, halitosis, and caries. Plaque matrix is mostly formed by protein (30%) and polysaccharide complexes. Thus, an alternative enzyme solution as denture cleanser is required for hydrol...
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| Main Authors: | , , |
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| Format: | Article PeerReviewed |
| Language: | Indonesian English |
| Published: |
Faculty of Dental Medicine, Universitas Airlangga
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/69118/1/per%20lutfi%205.pdf http://repository.unair.ac.id/69118/2/Jurnal%205.pdf http://repository.unair.ac.id/69118/ https://e-journal.unair.ac.id/MKG/article/view/1877 http://dx.doi.org/10.20473/j.djmkg.v49.i2.p81-86 |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian English |
| Summary: | Background: Accumulation of denture plaque can lead to pathological changes in oral mucosa, such as denture stomatitis, halitosis, and caries. Plaque matrix is mostly formed by protein (30%) and polysaccharide complexes. Thus, an alternative enzyme solution as denture cleanser is required for hydrolysis of denture plaque. Papain is a proteolytic enzyme hydrolyzing proteins, while β-1,3-glucanase is a hydrolase enzyme hydrolyzing polysaccharides. Purpose: This study aimed to analyze the differences of effectiveness of ß-1,3-glucanase Vigna unguiculata enzyme and papain Carica papaya enzyme in hydrolysis of denture plaque. Method: This research was a laboratory experimental research with post test only control group design. After using denture for 24 hours, the denture was soaked in a solution of 100 ml PBS, papain enzyme, and β 1-3 glucanase enzyme at a concentration of 0.5 mg/ml, 1 mg/ml, and 2 mg/ml for 10 minutes. The solution from plaque hydrolysis was soaked in PBS and vortex enzyme for 2 minutes, then soaked in ice water for 15 minutes, and centrifuged at 3000 rpm 5-10º for 10 minutes. The supernatant was separated and analyzed. Turbidity readings then were performed in spectrofotometer with a wavelength of 480 nm. Result: 2 mg/ml of ß-1,3 glucanase enzyme generated the highest values of hydrolysis with a mean percentage of 68.77% compared to papain enzyme (44.86 %). The lowest values of hydrolysis were generated by PBS with a mean percentage of 3.24%. Conclusion: ß-1,3-glucanase enzyme is more effective in hydrolysis of denture plaque than papain enzyme.
Keywords: Papain; ß-1,3-glucanase; denture plaque |
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