DETEKSI Blastocystis sp. DENGAN BERBAGAI PEWARNAAN DAN MEDIUM KULTUR PADA SUGAR GLIDER (Petaurus breviceps) DI SURABAYA PENELITIAN EKPLORATIF LABORATORIK
The biggest problem for veterinarian were the number of sudden death cases in the sugar glider. This was underlie the earlier preliminary research, and further research aimed to determine the effectiveness of staining and culture medium in diagnosing Blastocystis sp. on a sugar glider. The sample...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/70644/1/TPKMV.%2010-18%20Nat%20d%20Abstrak.pdf http://repository.unair.ac.id/70644/2/TPKMV.%2010-18%20Nat%20d.pdf http://repository.unair.ac.id/70644/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | The biggest problem for veterinarian were the number of sudden death cases
in the sugar glider. This was underlie the earlier preliminary research, and further
research aimed to determine the effectiveness of staining and culture medium in
diagnosing Blastocystis sp. on a sugar glider. The sample of this research were fresh
stool from 100 of sugar glider in Surabaya. The fresh stool sample directly stained
using Iodine, Methylene Blue, and Giemsa. Further, the samples were cultured on
simple medium and RPMI 1640. Medium was observed daily to see the morphology
of Blastocystis sp. The number of prevalence was very high rate of 100% with
Methylene Blue and Giemsa staining, 94% prevalence of Blastocystis sp. with
Iodine staining. The fresh stool sample were successfully cultured on RPMI 1640
for 5 days and simple medium for 6 days. The size of Blastocystis sp. was 0,38 –
2,95 μm (average 1,46 μm). The staining method that effective was staining with
Methylene Blue which economical, easy to do, effective in terms of time, protozoa
are clearly stained, and could be distinguished from other microorganisms.
Blastocystis sp. that found on sugar glider commonly smaller than Blastocystis sp.
that found on dog, cat, and Blastocystis hominis on human, but morphologically
was same with B. hominis. Vacuolar form was predominated found in the fresh
stool sample of sugar glider. |
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