DETEKSI ANTIMICROBIAL RESISTANCE PADA BAKTERI CITROBACTER FREUNDII YANG DIISOLASI DARI SWAB HEWAN PENGHASIL PANGAN PENELITIAN EKSPLORATIF LABORATORIS
The aim of this study was to detect blaTEM gene which the agent of antibiotic resistance in Citrobacter freundii isolated from rectal swab in dairy cattle, beef cattle, broiler chicken, catfish and milkfish. A total samples were 45 samples which rectal swab taken from 9 locations and 5 samples ta...
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| Main Author: | |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/70663/1/TPKMV.%2003-18%20Her%20d%20Abstrak.pdf http://repository.unair.ac.id/70663/2/TPKMV.%2003-18%20Her%20d.pdf http://repository.unair.ac.id/70663/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | The aim of this study was to detect blaTEM gene which the agent of
antibiotic resistance in Citrobacter freundii isolated from rectal swab in dairy
cattle, beef cattle, broiler chicken, catfish and milkfish. A total samples were 45
samples which rectal swab taken from 9 locations and 5 samples taken per
location. Rectal swab beef cattles from Krian, dairy cattles from Kaliwaron and
Bendul Merisi, broilers from Wonokromo, Keputran, Pacar Keling, catfishes
(Pangasius hypophthalmus) from Sedati and Pabean and milkfish (Chanos
chanos) from Sidoarjo. Then, samples were isolated with selective media and
biochemical tests. Antibiotic Resistance test was using the disc diffusion against
20 μg amoxycillin, 20/10 μg amoxycillin-clavulanic acid, 10/10 μg ampicillinsulbactam,
30 μg cefotaxime, 30 μg ceftazidime, 23.75/1,25 ug sulfametaxoletrimethoprim,
and 30 μg tetracycline and then detection of blaTEM gene used PCR.
The Result showed that seven samples were positive Citrobacter freundii of
broiler from Keputran (AK-5), broiler from Wonokromo (AW-1), dairy cattle
from Bendul Merisi (SB-3 ), dairy cattle from Krian (SPT.K-1) and milkfish from
Sidoarjo (IBS-1, IBS-2, IBS-4). Samples code AK-5, AW-1, SB-3 resistant to
ampicillin-sulbactam and tetracycline but SPT.K-1, IBS-1, IBS-2, IBS-4 resistant
to amoxicillin and ampicillin-sulbactam. Those seven samples were positive
blaTEM gene with 445 bp size used PCR. |
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