LAMA PENYINARAN PHOTODYNAMIC THERAPY TERHADAP JUMLAH BAKTERI LACTOBACILLUS ACIDOPHILUS (Penelitian Eksperimental Laboratoris)
Background Lactobacillus acidophilus is the one of etiological agents for dental caries and dominant in the deep carious lesion. Lactobacillus acidophilus also has been identified in persistent root canal infection and also related to the failure of endodontic treatment. Photodynamic therapy is a...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/70971/2/abstrak.pdf http://repository.unair.ac.id/70971/1/full%20text.pdf http://repository.unair.ac.id/70971/ |
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| Institution: | Universitas Airlangga |
| Language: | English English |
| Summary: | Background Lactobacillus acidophilus is the one of etiological agents for dental
caries and dominant in the deep carious lesion. Lactobacillus acidophilus also has
been identified in persistent root canal infection and also related to the failure of
endodontic treatment. Photodynamic therapy is a therapeutic process involving
the combination of a nontoxic photosensitizers and a light source. The excited
photosensitizer reacts with Reactive Oxygen Species (ROS), which induce injury
and death of microorganism. Purpose. The aim of this study is to prove the effect
of irradiation time of photodynamic therapy to the number of Lactobacillus
acidophilus. Methods. 42 Eppendorf tube was treated with 0,5 ml Lactobacillus
acidophilus distributed into 7 groups. Group I as the control group received no
treatment. Group II, III, IV, V, VI and VII was treated with combination of 0,5 ml
toluidine blue O (TBO) as photosensitizer and 630 nm fotoactivated (Fotosan®)
exposure time for 10 s, 20 s, 30 s, 40 s, 50s and 60 s. Then stored in an incubator
37ºC for 48 hours. And after that, counting the number of bacterial colonies in
CFU. Result. There were significant different of the number Lactobacillus
acidophilus in CFU between irradiation time. Conclusion. The longer of
photodynamic therapy irradiation the less number of life Lactobacillus
acidophilus. At 50 s and 60 s irradiation no one Lactobacillus acidophilus was
found. |
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