MEKANISME HAMBATAN PEMBENTUKAN SEL TRANSFORM PADA SEL EPITEL SKUAMOSA RONGGA MULUT AKIBAT LATIHAN RENANG INTENSITAS SEDANG (Pada Mus musculus strain Swiss Webster Yang Dipapar Benzopyrene)
Cancer is a serious problem in many parts of the world, head and neck cancer ranks 6th cancer in humans. About 95% of oral cancer is squamous cell carcinoma, is currently widely attacked in the age group under 40 years. Smoking is a risk factor because in cigarettes contained chemicals that are c...
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | English English |
Published: |
2018
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Subjects: | |
Online Access: | http://repository.unair.ac.id/71179/1/abstrak.pdf http://repository.unair.ac.id/71179/2/full%20text.pdf http://repository.unair.ac.id/71179/ |
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Institution: | Universitas Airlangga |
Language: | English English |
Summary: | Cancer is a serious problem in many parts of the world, head and neck cancer
ranks 6th cancer in humans. About 95% of oral cancer is squamous cell carcinoma, is
currently widely attacked in the age group under 40 years. Smoking is a risk factor
because in cigarettes contained chemicals that are carcinogenic, ie benzopyrene that
trigger DNA mutation, that body responded to repair by GADD45 and Hsp70, or
through apoptosis by wild p53, Bax and Bcl-2. Physical exercise has been shown to
have positive effect on the body’s systems, but there has been no study on the use of
exercise to decrease of risk of oral squamous cell carcinoma at an early stage. The
purpose of this study is explained the mechanism of inhibition transform cells
forming of oral squamous cell epithelial due to swimming exercise of moderate
intensity. Methods were : animals mice, Mus musculus strain Swiss Webster, male,
age 2 months, BW 25-35 g, a total of 18 were divided into 3 groups. K1: mice
brought into contact with water (gup), within 70% maximal swimming ability
(MSA), 3 times a week for 12 weeks; at week 5th, 0.04 ml of oleum olivarum
induced on the upper right buccal mucosa, 3 times a week for 4 weeks. K2 : same
with K1, at week 5th, induced by benzopyrene 0.08 mg/0.04 ml oleum olivarium. K3:
mice was given a moderate intensity of swimming exercise with load 3% of BW,
within 70% MSA, 3 times a week, for 12 weeks; at week 5th, same with K2. On the
first day of week 13th, mice anesthetized with ether, cutted the tumor tissue to the
examination of expression GADD45, Hsp70, wild p53, p53 mutant, Bax , Bcl-2, with
IHC methods; also counted the number of transform cells by HPA examination. Then
the mice were sacrificed. Result : There were no difference among the three groups
on GADD45 (p = 0.611) and Hsp70 (p = 0.874). There were significant differences
among the three groups on wild p53 (p = 0.000), p53 mutant (p = 0.000), Bax (p =
0.000), Bcl-2 (p = 0.000), and transform cells (p = 0.000). The conclusion of this
study was the mechanism of inhibition transform cell forming of oral squamous cell
epithelial due to swimming exercise of moderate intensity was through inhibition of
wild p53 decreased, inhibition of p53 mutant increased, increased of Bax and
decreased of Bcl-2. |
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