PERAN ENDOTHELIAL PROGENITOR CELLS (EPCs) DALAM PERUBAHAN POLARISASI MAKROFAG DENGAN FENOTIP M1 DAN M2 PADA MENCIT MODEL DIABETES MELLITUS
Background: Diabetes Mellitus (DM) is a disease that threatens global public health with their chronic complication, such as Diabetic Foot Ulcer (DFU). There are more macrophage phenotype M1 than M2 on DFU, that contribute to proinflamatory cytokines secretion, and there is an alteration of EPCs...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/71194/1/abstrak.pdf http://repository.unair.ac.id/71194/2/full%20text.pdf http://repository.unair.ac.id/71194/ |
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| Institution: | Universitas Airlangga |
| Language: | English English |
| Summary: | Background: Diabetes Mellitus (DM) is a disease that threatens global public
health with their chronic complication, such as Diabetic Foot Ulcer (DFU). There
are more macrophage phenotype M1 than M2 on DFU, that contribute to proinflamatory
cytokines secretion, and there is an alteration of EPCs in DM that
should have the ability of angiogenesis and neovascularization, so the wound
healing process in DM patient is slower than non-DM patient.
Objective: To prove in vitro role of Bone marrow (BM) derived EPCs in
polarization change of macrophage phenotype M1 and M2 in DM mice model
Methods: Peritoneal macrophages were co-cultured with EPCs and examined the
M1 and M2 markers by FACScalibur and qRT-PCR. The result would be
compared with the examination co-culture without EPCs.
Result: By FACScalibur examination, the results after co-culture with EPCs was
the M1 surface marker in control group were significantly decreased, namely
CD11c (p<0,0001), while M2 surface marker proved significantly increased, that
were CD206 (p<0,0001) and MGL1 (p<0,0001). The same results happened in
DM mice model group, that were CD11c (p<0,0001), CD206 (p=0,0445) and
MGL1 (p=0,0220). By qRT-PCR examination, the results after co-culture with
EPCs were the M1 genetic expression in DM mice model were significantly
decreased, namely CD11c (p<0,0001), TNF alpha (p=0,0002), IRF5 (p<0,0001),
while the M2 genetic expression proved significantly increased that were CD206
(p<0,0001), MGL1 (p=0,0002), Ym1 (p=0,0057), Arg1 (p=0,0234) and IL10
(p=0,0020). |
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