PENGARUH PEMBERIAN LISAT PLATELET RICH FIBRIN TERHADAP TINGKAT PROLIFERASI PADA KULTUR SEL PUNCA EPITEL LIMBUS YANG DIPAPAR NATRIUM HIDROKSIDA

• Main Author: Wahyu Endah Prabawati,, NIM011318016305
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian
• Published: 2018
• Subjects: R Medicine (General)
• Online Access: http://repository.unair.ac.id/71241/1/TKKli.%2005-18%20Pra%20p%20Abstrak.pdf; http://repository.unair.ac.id/71241/2/TKKli.%2005-18%20Pra%20p.pdf; http://repository.unair.ac.id/71241/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

Purpose: The purpose of this study was to investigate the effect of platelet rich fibrin lysates on limbal stem cell proliferation which was exposed by alkali Methods: Confluent rabbit limbal stem cells wounded using 20μL of 0,00625 M sodium hydroxide were treated with platelet rich fibrin lysates (PRF) (0,5, and 10%). PRF lysates was prepared from rabbit peripheral blood according to Choukroun’s method without using anticoagulant and foreign factors for platelet activation. The proliferation of limbal stem cells were measured by an 3-(4,5- dimethylthiazol-2-yl)-2,50diphenyl tetrazolium bromide (MTT) colorimetric assay at 24,48, 72 hours after exposed by sodium hydroxide. Results: Proliferation significantly increase on limbal stem cell with PRF lysates 5% (p=0000) and 10% (p=0,000) group compared with control. There is no significant difference between PRF lysates 5% and 10% (p=0,98). The highest proliferationof limbal stem cell is PRF lysates5% group after 48 hours (100,24%) Conclusion: PRF stimulates limbal stem cell proliferation in chemical trauma caused by natrium hydroxide model. PRF repairs limbal stem cell niche and influences limbal stemness. The present findings warrant further research on PRF as a novel alternative treatment for limbal stem cells deficiency.