PENGARUH PEMBERIAN LISAT PLATELET RICH FIBRIN TERHADAP TINGKAT PROLIFERASI PADA KULTUR SEL PUNCA EPITEL LIMBUS YANG DIPAPAR NATRIUM HIDROKSIDA
Purpose: The purpose of this study was to investigate the effect of platelet rich fibrin lysates on limbal stem cell proliferation which was exposed by alkali Methods: Confluent rabbit limbal stem cells wounded using 20μL of 0,00625 M sodium hydroxide were treated with platelet rich fibrin lysate...
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | Indonesian Indonesian |
Published: |
2018
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Subjects: | |
Online Access: | http://repository.unair.ac.id/71241/1/TKKli.%2005-18%20Pra%20p%20Abstrak.pdf http://repository.unair.ac.id/71241/2/TKKli.%2005-18%20Pra%20p.pdf http://repository.unair.ac.id/71241/ http://lib.unair.ac.id |
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Institution: | Universitas Airlangga |
Language: | Indonesian Indonesian |
Summary: | Purpose: The purpose of this study was to investigate the effect of platelet rich
fibrin lysates on limbal stem cell proliferation which was exposed by alkali
Methods: Confluent rabbit limbal stem cells wounded using 20μL of 0,00625 M
sodium hydroxide were treated with platelet rich fibrin lysates (PRF) (0,5, and
10%). PRF lysates was prepared from rabbit peripheral blood according to
Choukroun’s method without using anticoagulant and foreign factors for platelet
activation. The proliferation of limbal stem cells were measured by an 3-(4,5-
dimethylthiazol-2-yl)-2,50diphenyl tetrazolium bromide (MTT) colorimetric
assay at 24,48, 72 hours after exposed by sodium hydroxide.
Results: Proliferation significantly increase on limbal stem cell with PRF lysates
5% (p=0000) and 10% (p=0,000) group compared with control. There is no
significant difference between PRF lysates 5% and 10% (p=0,98). The highest
proliferationof limbal stem cell is PRF lysates5% group after 48 hours (100,24%)
Conclusion: PRF stimulates limbal stem cell proliferation in chemical trauma
caused by natrium hydroxide model. PRF repairs limbal stem cell niche and
influences limbal stemness. The present findings warrant further research on PRF
as a novel alternative treatment for limbal stem cells deficiency. |
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