PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS
Justicia gendarussa Burm.f contains several flavonoids a of them is ‘gendarusin A’ that has the ability as antifertility with mechanism is to prevent spermatozoa penetration by inhibiting the activity of hyaluronidase spermatozoa enzyme. Hyaluronidase is one of the enzymes found on the outside o...
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id-langga.719152018-04-20T01:02:28Z http://repository.unair.ac.id/71915/ PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS KHUSNUL KHOTIMAH, 051311131167 RS1-441 Pharmacy and materia medica Justicia gendarussa Burm.f contains several flavonoids a of them is ‘gendarusin A’ that has the ability as antifertility with mechanism is to prevent spermatozoa penetration by inhibiting the activity of hyaluronidase spermatozoa enzyme. Hyaluronidase is one of the enzymes found on the outside of the spermatozoa that plays a role for the penetration of the cumulus oophorus by hydrolyzing hyaluronic acid in the process of fertilization. This study aim to find out the effect of 70% fractionated ethanol extract of Justicia gendarussa Burm.f leaf at dose of sub chronic toxicity study on the activity of rat’s hyaluronidase spermatozoa enzyme. The methode used was microplate assay. Hyaluronidase activities can be detected from residues of hyaluronic acid that were not hydrolyzed by hyaluronidase and precipitated with 10% cetilpiridinium cloride that gave the absorbance in the λ 595 nm. Moreover, in this study, Bradford method is utilized in order to find the specific activity of hyaluronidase enzyme. Specific activity of hyaluronidase enzyme can be known from catalytic activity per miligram of protein. In the experiment process, it was showed that the average catalytic activity of the hyaluronidase spermatozoa enzyme on group of rats which were not given 70% ethanol extract of Justicia gendarussa Burm.f is as 0,778.10-6 unit/1 million spermatozoa, while on group of rats which were given 70% ethanol extract of Justicia gendarussa Burm.f with 40 mg , 200 mg, and 1000 mg doses respectively are as 0,746.10-6 unit /1 million spermatozoa, 0,696.10-6 unit/1 million spermatozoa, and 0,685.10-6 unit/1 million spermatozoa. To conclusion, based on the results of the study, there is a decrease of catalytic activity and the average of rat spermatozoa. 2018 Thesis NonPeerReviewed text id http://repository.unair.ac.id/71915/1/FF.FT.%2005-18%20Kho%20p%20Abstrak.pdf text id http://repository.unair.ac.id/71915/2/FF.FT.%2005-18%20Kho%20p.pdf KHUSNUL KHOTIMAH, 051311131167 (2018) PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS. Skripsi thesis, UNIVERSITAS AIRLANGGA. http://lib.unair.ac.id |
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RS1-441 Pharmacy and materia medica KHUSNUL KHOTIMAH, 051311131167 PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS |
description |
Justicia gendarussa Burm.f contains several flavonoids a of
them is ‘gendarusin A’ that has the ability as antifertility with mechanism
is to prevent spermatozoa penetration by inhibiting the activity of
hyaluronidase spermatozoa enzyme. Hyaluronidase is one of the enzymes
found on the outside of the spermatozoa that plays a role for the
penetration of the cumulus oophorus by hydrolyzing hyaluronic acid in
the process of fertilization. This study aim to find out the effect of 70%
fractionated ethanol extract of Justicia gendarussa Burm.f leaf at dose of
sub chronic toxicity study on the activity of rat’s hyaluronidase
spermatozoa enzyme. The methode used was microplate assay.
Hyaluronidase activities can be detected from residues of hyaluronic acid
that were not hydrolyzed by hyaluronidase and precipitated with 10%
cetilpiridinium cloride that gave the absorbance in the λ 595 nm.
Moreover, in this study, Bradford method is utilized in order to find the
specific activity of hyaluronidase enzyme. Specific activity of
hyaluronidase enzyme can be known from catalytic activity per miligram
of protein. In the experiment process, it was showed that the average
catalytic activity of the hyaluronidase spermatozoa enzyme on group of
rats which were not given 70% ethanol extract of Justicia gendarussa
Burm.f is as 0,778.10-6 unit/1 million spermatozoa, while on group of rats
which were given 70% ethanol extract of Justicia gendarussa Burm.f
with 40 mg , 200 mg, and 1000 mg doses respectively are as 0,746.10-6
unit /1 million spermatozoa, 0,696.10-6 unit/1 million spermatozoa, and
0,685.10-6 unit/1 million spermatozoa. To conclusion, based on the
results of the study, there is a decrease of catalytic activity and the
average of rat spermatozoa. |
format |
Theses and Dissertations NonPeerReviewed |
author |
KHUSNUL KHOTIMAH, 051311131167 |
author_facet |
KHUSNUL KHOTIMAH, 051311131167 |
author_sort |
KHUSNUL KHOTIMAH, 051311131167 |
title |
PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia
gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP
AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS |
title_short |
PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia
gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP
AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS |
title_full |
PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia
gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP
AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS |
title_fullStr |
PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia
gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP
AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS |
title_full_unstemmed |
PENGARUH EKSTRAK ETANOL 70% TERFRAKSINASI DAUN Justicia
gendarussa Burm.f PADA STUDI TOKSISITAS SUBKRONIK TERHADAP
AKTIVITAS ENZIM HIALURONIDASE SPERMATOZOA TIKUS |
title_sort |
pengaruh ekstrak etanol 70% terfraksinasi daun justicia
gendarussa burm.f pada studi toksisitas subkronik terhadap
aktivitas enzim hialuronidase spermatozoa tikus |
publishDate |
2018 |
url |
http://repository.unair.ac.id/71915/1/FF.FT.%2005-18%20Kho%20p%20Abstrak.pdf http://repository.unair.ac.id/71915/2/FF.FT.%2005-18%20Kho%20p.pdf http://repository.unair.ac.id/71915/ http://lib.unair.ac.id |
_version_ |
1681149971861078016 |