Hylocereus Polyrhizus Peel Ethanol Extract - The Potential Effect to Tumor Necrosis Factor - A, Macrophage, and Matrix Metalloproteinase - 9 in Endometriosis Mice

• Main Authors: Hapsari, Anindya, Hendarto, Hendy, Widjiati
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Ektodermal Displazi Grubu, Ectodermal Dysplasia Turkey 2017
• Subjects: SF600-1100 Veterinary medicine; Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
• Online Access: http://repository.unair.ac.id/72137/18/Bukti%20C%2009%20Hylocereus%20Polyrhizus%20Peel%20Ethanol%20Extract%20-%20The%20Potential%20....pdf; http://repository.unair.ac.id/72137/3/Peer%20Review%20Bukti%20C%2009%20Hylocereus%20Polyrhizus%20Peel%20Ethanol%20Extract.pdf; http://repository.unair.ac.id/72137/1/Bukti%2009%20Hylocereus%20Polyrhizus%20Peel%20Ethanol%20Extract-%20the%20Potential%20Effect%20to%20Tumor%20Necrosis%20Factor-%CE%91%2C%20Macrophage%2C%20and%20Matrix%20Metalloproteinase-9%20in%20Endometriosis%20Mice%20%284%29.pdf; http://repository.unair.ac.id/72137/; http://www.jidmr.com/journal/wp-content/uploads/2017/12/41.M17_409_Widjiati_Alexander_Nugraha.pdf
• Institution: Universitas Airlangga

On endometriosis, macrophage and TNF-α was found in higher concentration. Tumor Necrosis Factor-α activates NF-κB pathway and increase the expression of MMP-9’s gen. NF-κB pathway can be blocked by Hylocereus polyrhizus peel. The objective of this study was to know the effect of Hylocereus polyrhizus peel ethanol extract at dose 0,25; 0,5 mg/gram BW/day; and 1 mg/gram on TNF-α concentration, number of macrophage, and MMP-9 expression on mouse model of endometriosis. This study was a laboratory experimental research. Thirty female mice were used as samples and divided into 5 groups: 1 positive control, 1 negative control, and 3 treatment groups. Positive control and treatment groups were induced as model of endometriosis for 14 days. The next 14 days, Na-CMC 0,5% was given to both control groups, while Hylocereus polyrhizus peel ethanol extract dose 0,25; 0,5 mg/gram BW/day; and 1 mg/gram were given to treatment groups orally. Peritoneum fluid and endometriosis lesion were examined. There were significantly differences on TNF-α concentration (p=0.021), number of macrophage (p=0.00), and MMP-9 expression (p=0.002) among control groups and treatment groups. TNF-α concentrations were higher on treatment groups. Hylocereus polyrhizus peel ethanol extract can be used as alternative therapy for attenuating the alteration of macrophage and MMP-9 in endometriosis disease