Effect of Rat Bone Marrow Stem Cell Administration to Rats (Rattus novergicus) Using Infertile Model on Oocyte Apoptosis

• Main Author: Widjiati
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Asian Association of Veterinary Anatomist 2015
• Subjects: SF600-1100 Veterinary medicine; Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
• Online Access: http://repository.unair.ac.id/72280/1/Peer%20Review%20Bukti%20C%2025%20Effect%20of%20Rat%20Bone%20Marrow%20Stem%20Cell%20Administration%20to%20....pdf; http://repository.unair.ac.id/72280/12/Bukti%20C%2025%20Effect%20of%20Rat%20Bone%20Marrow%20Stem%20Cell%20Administration%20to%20Rats%20%28Rattus%20novergicus%29....pdf; http://repository.unair.ac.id/72280/2/Bukti%2025%20Effect%20of%20Rat%20Bone%20Marrow%20Stem%20Cell%20Administration%20to%20Rats%20%28Rattus%20norvegicus%29%20Using%20Infertile%20Model%20on%20Oocyte%20Apoptosis%20%283%29.pdf; http://repository.unair.ac.id/72280/; http://journal.ipb.ac.id/index.php/veteriner/article/viewFile/18701/13203
• Institution: Universitas Airlangga

NTRODUCTION Many factors can cause infertility of cattle, and endocrine abnormality is the most frequently found factor. High infertility rate will be of disadventage because it will decrease the reproductivity and productivity of cattle. Nowdays, the utilization of stem cell to cure various diseases is increasing as Rat Bone Marrow Stem Cell (RBMSC) is able to express genes or certain cells in the body. In infertility case, Rat Bone M arrow Stem Cell administration can improve folliculogenesis process and the quality of oocyte ovulated. The objective of the study is to find out the effect of RBMSC administration on oocyte apoptosis of rats. The significance of the study is a new innovation to utilize stem cells for reproductive disorders. MATERIALS DAN METHODS The study was an experimental research with post test only control group design using 32 rats of infertile model randomly divided into 2 groups, that is, Group 1 (Control), a group of rats of infertile model given minimum engle medium (MEM) as RBMSC solvent and Group II (Treatment), a group of rats of infertile model given RBMSC therapy with a dosage of 1.10 7/rat. Oocyte apoptosis was evaluated using tunnel coloring