Effect of hematopoietic stem cell on tumor necrosis factor-α expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice

• Main Authors: Widjiati, Kuncorojakti, Suryo, Hendarto, Hendy, Hendrawan, Viski Fitri, Aulanni’am
• Format: Article PeerReviewed
• Language: English; Indonesian; English
• Published: Elsevier BV 2017
• Subjects: Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
• Online Access: http://repository.unair.ac.id/72382/18/Bukti%20C%2005%20Effect%20of%20Hematopoietic%20stem%20cell%20on%20tumor%20necrosis%20factor-a%20expression%2C%20spiral%20artery%20.....pdf; http://repository.unair.ac.id/72382/3/Peer%20Review%20Bukti%20C%2005%20Effect%20of%20Hematopoietic%20stem%20cell%20.....pdf; http://repository.unair.ac.id/72382/1/Bukti%2005%20Effect%20of%20hematopoietic%20stem%20cell%20on%20tumor%20necrosis%20factor-%E6%AF%A9expression%2C%20spiral%20artery%20remodeling%20and%20placental%20apoptosis%20in%20lead-exposed%20pregnant%20mice%20%282%29.pdf; http://repository.unair.ac.id/72382/; http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=4;spage=158;epage=163;aulast=Widjiati,
• Institution: Universitas Airlangga

Objective: To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF- a expression, spiral artery remodeling, and placental apoptosis. Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment; Group 2, 1/12 LD50 of lead and Aquadest; Group 3, 1/16 1-DSO of lead and Aquadest, Group 4, 1/12 LDso of lead and 1 X 107 HSC; and group 5, 1/16 1-DSO of lead and 1 X 107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF- a expression and the apoptosis index. The data from all five groups were compared using the ANOVA test. Results: TNF- a expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<O.OI). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the gimps of 1/16 1-DSO of lead followed HSC (P>0.05). Conclusions: In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF- a expression, spiral artery remodeling, and placental apoptosis index but in the group of I /1 6 LDs, of lead and HSC treatment, apoptosis index has no differences with those of control group