PENGARUH PENAMBAHAN TEPUNG JANGKRIK KALUNG (Gryllus bimaculatus) DALAM PAKAN MENCIT (Musmusculus) JANTAN TERHADAP BERAT TESTIS, DIAMETER TUBULUS SEMINIFERUS DAN JUMLAH SEL LEYDIG
This research’s purpose is to see the effect of ‘Kalung’ cricket’s (Gryllus bimaculatus) flouraddition in male mice (Mus musculus) feed toward the testes’ weight, seminiferous tubules’ diameter, and leydig cells’ amount. The effect of cricket flour was tested on 20 mice which are randomly mixed a...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/72777/1/ABSTRAK_KH.118%2018%20Pra%20p.pdf http://repository.unair.ac.id/72777/2/FULLTEXT_KH.118%2018%20Pra%20p.pdf http://repository.unair.ac.id/72777/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | English English |
| Summary: | This research’s purpose is to see the effect of ‘Kalung’ cricket’s (Gryllus
bimaculatus) flouraddition in male mice (Mus musculus) feed toward the testes’
weight, seminiferous tubules’ diameter, and leydig cells’ amount. The effect of
cricket flour was tested on 20 mice which are randomly mixed at 4 treatments. K
(control) was given standard feed, P1 was given standard feed with addition of 5%
cricket flour, P2 with addition of 10% cricket flour, and P3 with addition of 20%
cricket flour. Treatment was done for 45 days, then the mice were sacrificed. Testes
sample was taken by surgery, testes’ weight were weighed using 0.1 analytical
scale, then proceed with the preparation of histologic preparations of the
seminiferous tubules to see the seminiferous tubules’ diameter and leydig cells’
amount. Measurement of seminiferous tubules’ diameter using Trinocular
microscope with 100x magnification with NIS-Element High Contain Analysis
software. Calculation of leydig cells’ amount using Optilab with Image Raster
software with 400x magnification. The results were analyzed using Analysis of
Variant (ANOVA) and continued with Duncan range test of obtian average value
and standard deviation. The weight of testes are 0.09±0.016 (K), 0.13±0.015 (P1),
0.10±0.031 (P2), 0.05±0.023 (P3). Seminiferous tubules’ diameter are
174.32±8.032 (K), 204.27±18.004 (P1), 182,68 ±10.233 (P2), 139.03±13.042 (P3).
Leydig cells’ amount are 11.78±2.072 (K), 16.40±2.821 (P1), 11.82±1.428 (P2),
8.26±1.342 (P3) which shows significant differences (P<0.05). Conclusion is, the
addition of 5% cricket’s flour dose is the optimal dose to increase the testes’
weight, seminiferous tubules’ diameter, and leydig cells’ amount. |
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