PENGARUH KONSENTRASI O2 DAN JUMLAH PASASE TERHADAP VIABILITAS, APOPTOSIS, STEMNESS, DAN SENESCENCE PADA KULTUR SEL PUNCA MESENSIMAL SUMSUM TULANG (STSPM) KELINCI NEW ZEALAND
Background: Oxygen concentration is an important component in the niche as it contributes to the development and function of bone marrow mesenchymal stem cells (bmMSC). Normoxia culture results in DNA damage and apoptosis of stem cell. Hypoxic culture studies have different results because the me...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/72911/1/Dis.K.%2023-18%20Bum%20p%20Abstrak%20pdf.pdf http://repository.unair.ac.id/72911/2/Dis.K.%2023-18%20Bum%20p%20pdf.pdf http://repository.unair.ac.id/72911/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Background: Oxygen concentration is an important component in the niche as it contributes
to the development and function of bone marrow mesenchymal stem cells (bmMSC). Normoxia
culture results in DNA damage and apoptosis of stem cell. Hypoxic culture studies have
different results because the mechanism is unclear. Aim: To explained decreasing of
apoptosis, senescence and increasing of viability and stemness due to the O2 concentration
and passage of bmMSC. Methods: bmMSC are taken from the bone marrow of the rabbit then
cultured hypoxia (O2 1%), normoxia (O2 21%), and normo-hypoxic cultures up to 10 passage.
At 2,4 passage (early passage) and 7, 10 passage 7, 10 (late passage) investigate viability,
apoptosis, stemness, and senescence. Results: Hypoxia was significantly different (p <0.05)
to maintaining viability and stemness also decreased the number of apoptosis and senescence
while the passage did not differ significantly (p> 0.05) to decreased oct4 and senescence
expression in late passage. In late passsage, normoxia cultures occur more amino acid
mutations of oct4 than hypoxia cultures and normo-hypoxic cultures. Hypoxia can induced
stemness by increasing HIF- expression and oct4 expression while the passage is associated
with decreasing oct4 expression. Conclusions: Hypoxia can increasing viability and stemness
of bmMSC and decreasing of apoptosis and stemness of bmMSC. Hypoxia is capable of up
regulation oct4 after decreasing in normoxia culture. |
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