Physical Characteristics of Liposomal Formulation dispersed in HPMC Matrix and Freeze-Dried using Maltodextrin and Mannitol as Lyoprotectan
Background: The present study aims to design formulation of liposomes that are well-preserved during freeze-drying. The combination of Hydroxy Propyl Methyl Cellulose (HPMC) as dispersion matrix and lyoprotectants; maltodextrin or mannitol, was employed to prevent aggregation and/or recrystalliz...
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| Main Authors: | , , |
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| Format: | Article PeerReviewed |
| Language: | English English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/73331/1/C-10%20Artikel.pdf http://repository.unair.ac.id/73331/2/C-10%20Validasi%20dan%20Peer%20Reviewer.pdf http://repository.unair.ac.id/73331/ http://journals.tbzmed.ac.ir/PHARM/Manuscript/PHARM-23-285.pdf |
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| Institution: | Universitas Airlangga |
| Language: | English English |
| Summary: | Background: The present study aims to design formulation of liposomes that
are well-preserved during freeze-drying. The combination of Hydroxy Propyl
Methyl Cellulose (HPMC) as dispersion matrix and lyoprotectants;
maltodextrin or mannitol, was employed to prevent aggregation and/or
recrystallization. The obtained dry products were investigated in terms of their
physical characteristics.
Methods: Liposomes were prepared using thin film method and hydrated with
the lyoprotectant solution. The formed liposomes were mixed with HPMC gel
and freeze-dried. The obtained solid products were characterized using
Differential Scanning Calorimetry (DSC), X-Ray Diffraction (XRD), and
Scanning Electron Microscopy (SEM).
Results: The DSC thermograms of formulations with maltodextrin were
relatively homogenous, yet exhibiting meta-stable properties. In contrast, the
formulations using mannitol showed phase separation. These results were
confirmed by XRD data, in which formulations with maltodextrin showed no
intensive peaks, indicating amorphous solid while the formulations with
mannitol exhibited more intensive peaks, indicating the presence of crystalline
solids. The SEM images of both maltodextrin and mannitol-containing
formulations showed porous matrix with spherical liposomes trapped in the
matrices. The SEM images also correspond to the DSC and XRD data, where
crystalline solid existed in the mannitol-containing formula.
Conclusion: The developed liposomes formulation using combination of
HPMC matrix and maltodextrin showed potential in preserving liposomes
structure, contrary to those of using mannitol.
Keywords: Liposomes, Lyoprotectant, Freeze-Drying, Maltodextrin, Mannitol, HPMC |
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