PENGARUH DAN MEKANISME KERJA EKSTRAK ETANOL BUAH MERAH (PANDANUS CONOIDEUS LAM) TERHADAP APOPTOSIS SEL GRANULOSA OVARIUM MENCIT MODEL ENDOMETRIOSIS MELALUI ANALISIS EKSPRESI TNF, NF-kB, CASPASE3, HSP70 DAN SOD
AIM: Purpose of this study was to prove whether the administration of red fruit extract therapy can reduce the incidence of apoptosis in ovarian granulosa cells of mice endometriosis model. METHODS:This study used experimental research design, 27 mice of endometriosis model (BalB / c) was divide...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/74351/1/Dis.%20K.%2031-18%20Rat%20p%20Abstrak.pdf http://repository.unair.ac.id/74351/2/Dis.%20K.%2031-18%20Rat%20p.pdf http://repository.unair.ac.id/74351/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | AIM: Purpose of this study was to prove whether the administration of red fruit extract
therapy can reduce the incidence of apoptosis in ovarian granulosa cells of mice
endometriosis model.
METHODS:This study used experimental research design, 27 mice of endometriosis
model (BalB / c) was divided into 3 groups, each consist of 9 mice: normal mice group
group (K-), endometriosis model group received placebo (K+) and group of
endometriosis model of mice given red fruit extract (P). samples were taken from the
peritoneum to evaluate Tumor Necrosis Factor alpha (TNF) and Nuclear factor kappa
beta (NF-kB) and ovaries for caspase 3, Heat shock protein (HSP70) and Superoxide
dismutase (SOD).
RESULTS :Administration of red fruit extract decrease granulosa cell apoptosis. The
index of ovaries granulosa cell apoptosis in mice of K+ group was significantly higher
compared to P group (p <0.05). TNF and NF-kB of endometriosis mice given red
fruit extract was significantly lower than in the group endometriosis mice that received
placebo (p <0.05). The decrease of TNF do not have correlation with the decrease of
NF-kB (B=0,001;p=0,684). Caspase3 in the positive control group increased
significantly compared with the negative control group (p <0.05). The SOD expression
in P group was significantly higher than the K+ group (p <0.05). HSP 70 levels in the
K+ group were higher than in the P (p <0.05).
CONCLUSION : Red fruit ethanol extract decrease apoptosis of granulosa cells due
to endometriosis, through the mechanism of decreasing TNF and caspase3
expressions. There is increase of SOD, and decrease of NF-kB, TNF and HSP70 after
Red fruit ethanol extract administration. From pathway analysis shows that the
decrease of NF-kB expression is not correlate with decrease of TNF. And the increase
of SOD expression also not correlate with decrease of granulosa cell apoptotic index .
Administration of red fruit ethanol extract causing HSP70 expression decrease. The
decrease of apoptosis is not correlate with HSP70 expression decrease. |
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