PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar)
Objective: To investigate the effect of curcumin on GSH and GSSG level in hyperglycemia induced corneal tissue. Materials and Method: Corneal tissue obtained from hyperglycemia induced Wistar rats usingintraperitoneal injection of streptozotocin (STZ) (45 mg/kgBW) with and without exposure to cu...
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id-langga.747912018-10-17T13:10:48Z http://repository.unair.ac.id/74791/ PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar) MUHAMMAD RONY, NIM011218016302 RE Ophthalmology Objective: To investigate the effect of curcumin on GSH and GSSG level in hyperglycemia induced corneal tissue. Materials and Method: Corneal tissue obtained from hyperglycemia induced Wistar rats usingintraperitoneal injection of streptozotocin (STZ) (45 mg/kgBW) with and without exposure to curcumin(1 g/kgBW). The blood sugar checked within 72 hours after STZ administration. Control group is STZ and curcuminfree. After 6 weeks, GSH and GSSG levels were examined in each groupsby enzymelinked immunosorbent assay(ELISA). Result: The mean levels of GSH in group I, II, III were 144.18 micromol/L, 14.02 micromol/L, and 144.98 micromol/L respectively. The mean levels of GSSG in group I, II, III were 11.82 micromol/L, 141.40 micromol/L, and 11.09 micromol/L respectively. After we conducted statistical analysis in each group (control, STZ, STZ+curcumin), there were difference in GSH/GSSG level The results of GSH analysis between group I (control) on group II (STZ) and group II (STZ) on group III (STZ+Curcumin) (p=0,00; p=0.00). However,there were no significant difference on GSH/GSSG between group I (control) with group III (STZ+Curcumin) (p=0,92; p=0,72). Conclution:Curcuminincrease GSH/GSSG ratio in hyperglycemia induced corneal damage indicate that curcuminstimulates the increase of corneal antioxidant level and cell reepithelialization. Curcuminis suggested as alternative therapy for corneal epithelialization damage caused by hyperglycemia condition 2018 Thesis NonPeerReviewed text id http://repository.unair.ac.id/74791/1/PPDS.IKM.%2016-18%20Ron%20p%20Abstrak.pdf text id http://repository.unair.ac.id/74791/2/PPDS.IKM.%2016-18%20Ron%20p.pdf MUHAMMAD RONY, NIM011218016302 (2018) PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar). Thesis thesis, Universitas Airlangga. http://lib.unair.ac.id |
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RE Ophthalmology MUHAMMAD RONY, NIM011218016302 PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar) |
description |
Objective: To investigate the effect of curcumin on GSH and GSSG level in
hyperglycemia induced corneal tissue.
Materials and Method: Corneal tissue obtained from hyperglycemia induced
Wistar rats usingintraperitoneal injection of streptozotocin (STZ) (45 mg/kgBW)
with and without exposure to curcumin(1 g/kgBW). The blood sugar checked
within 72 hours after STZ administration. Control group is STZ and curcuminfree.
After 6 weeks, GSH and GSSG levels were examined in each groupsby enzymelinked
immunosorbent assay(ELISA).
Result: The mean levels of GSH in group I, II, III were 144.18 micromol/L, 14.02
micromol/L, and 144.98 micromol/L respectively. The mean levels of GSSG in
group I, II, III were 11.82 micromol/L, 141.40 micromol/L, and 11.09 micromol/L
respectively. After we conducted statistical analysis in each group (control, STZ,
STZ+curcumin), there were difference in GSH/GSSG level The results of GSH
analysis between group I (control) on group II (STZ) and group II (STZ) on group
III (STZ+Curcumin) (p=0,00; p=0.00). However,there were no significant
difference on GSH/GSSG between group I (control) with group III
(STZ+Curcumin) (p=0,92; p=0,72).
Conclution:Curcuminincrease GSH/GSSG ratio in hyperglycemia induced corneal
damage indicate that curcuminstimulates the increase of corneal antioxidant level
and cell reepithelialization. Curcuminis suggested as alternative therapy for corneal
epithelialization damage caused by hyperglycemia condition |
format |
Theses and Dissertations NonPeerReviewed |
author |
MUHAMMAD RONY, NIM011218016302 |
author_facet |
MUHAMMAD RONY, NIM011218016302 |
author_sort |
MUHAMMAD RONY, NIM011218016302 |
title |
PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar) |
title_short |
PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar) |
title_full |
PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar) |
title_fullStr |
PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar) |
title_full_unstemmed |
PENGARUH CURCUMIN TERHADAP KADAR GSH DAN GSSG PADA KORNEA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental padaTikus Wistar) |
title_sort |
pengaruh curcumin terhadap kadar gsh dan gssg pada kornea model tikus hiperglikemia (studi eksperimental padatikus wistar) |
publishDate |
2018 |
url |
http://repository.unair.ac.id/74791/1/PPDS.IKM.%2016-18%20Ron%20p%20Abstrak.pdf http://repository.unair.ac.id/74791/2/PPDS.IKM.%2016-18%20Ron%20p.pdf http://repository.unair.ac.id/74791/ http://lib.unair.ac.id |
_version_ |
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