EFEK ANTINOSISEPTIF EKSTRAK KLOROFORM DAN FRAKSI ETANOL BUAH Piper retrofractum Vahl. PADA MENCIT: KETERLIBATAN MEKANISME HAMBATAN COX-2
Analgesic compounds are widely used throughout the world but can cause some adverse effects. This issue encourages studies to find alternative medicine derived from plants. P.retrofractum extract has an analgesic effect on mice, but there is no further information on its antinociceptive mechanism...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/75260/1/TF.%2026-18%20Sya%20e%20Abstrak.pdf http://repository.unair.ac.id/75260/2/TF.%2026-18%20Sya%20e.pdf http://repository.unair.ac.id/75260/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Analgesic compounds are widely used throughout the world but can cause
some adverse effects. This issue encourages studies to find alternative medicine
derived from plants. P.retrofractum extract has an analgesic effect on mice, but
there is no further information on its antinociceptive mechanisms. One of the most
widely used tests to determine the antinociceptive effect is nociception induction
by injecting formalin in plantar areas of experimental animals. Intraplantar
injection of formalin causes recurrent syndrome of apparent nociceptive behavior
in two distinct phases.
In the present study, the effect of chloroform extract and ethanol 96%
fraction of P.retrofractum fruit on spontaneous pain behaviors and expression of
COX-2 were examined in mice. The method used is the induction of pain using
formalin injected at the plantar area of the hind paw of the mouse. Furthermore,
an immunohistochemical examination was performed to validate the expression of
COX-2.
Based on the one-way ANOVA statistical analysis followed by Tukey test,
there was a significant difference in the decrease of mice pain response and foot
edema between treatment group of chloroform extract and ethanol fraction with
negative control group (p <0,05). It appears that at the same dose containing
piperine, the expression of COX-2 in the ethanol fraction group was lower than
that of the chloroform extract group. These results indicate that there are other
compounds besides piperine that contained in Piper retrofractum which are also
responsible for provide analgesic activity. |
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