DETEKSI RESISTENSI ISOLAT Escherichia coli DARI AYAM BROILER DI KECAMATAN DLANGGU KABUPATEN MOJOKERTO TERHADAP AMIKACIN DAN CEFOTAXIME
The aim of this research was to determine resistance of sensitivity test in Eschercia coli from rectal swab of broiler. The first step of this research is to prepare the isolation of Escherichia coli. Samples were collected from 2 different broiler farm at Dlanggu, Mojokerto. This study were obta...
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| Main Author: | |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/76220/1/ABSTRACT.pdf http://repository.unair.ac.id/76220/2/KH%20142-18%20War%20d.pdf http://repository.unair.ac.id/76220/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | The aim of this research was to determine resistance of sensitivity test in
Eschercia coli from rectal swab of broiler. The first step of this research is to
prepare the isolation of Escherichia coli. Samples were collected from 2 different
broiler farm at Dlanggu, Mojokerto. This study were obtained 30 positive
Escherichia coli isolates from total 40 sample. Preparation of the isolation and
identification was confirmed by Mac Conkey Agar (MCA), Eosin Methylen Blue
Agar (EMBA), Gram staining, indole test and motility test on Sulfide Indol
Mootility (SIM), Methly Red Voges Proskauer (MR-VP) test, Citrate test on
Simons Citrate Agar (SCA), and Triple Sugar Iron Agar (TSIA) test. Escherichia
coli isolated were tested using the test sensitivity to antibiotics by the Kirby-Bauer
method. The results showed that the sample isolated Gram negative, coccobacil,
solitary or short chain on Gram staining, positive indole and motil on SIM, positif
MR, negatif VP, negative citrate and acid/acid, positif gas, negative H2S on TSIA.
The results showed that isolates of Escherichia coli, resistant to cefotaxime by
30% but still sensitive by 70% and to the amikacin that is 100% sensitive. |
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