PENGARUH KONSENTRASI HPC-L DAN HPMC 606 TERHADAP KARAKTERISTIK FISIK DAN PELEPASAN BOVINE SERUM ALBUMIN DARI MIKROSFER
The aim of this study was to investigate the preparation of microspheres as potential protein carriers. Bovine serum albumin (BSA) was chosen as a hydrophilic model protein due to its known structure and ease of quantitative assay. BSA was encapsulated within hydroxypropyl cellulose low viscosity...
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | Indonesian Indonesian |
Published: |
2018
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Subjects: | |
Online Access: | http://repository.unair.ac.id/76410/1/TF.%2035-18%20Sar%20p%20Abstrak.pdf http://repository.unair.ac.id/76410/2/TF.%2035-18%20Sar%20p.pdf http://repository.unair.ac.id/76410/ http://lib.unair.ac.id |
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Institution: | Universitas Airlangga |
Language: | Indonesian Indonesian |
Summary: | The aim of this study was to investigate the preparation of microspheres as
potential protein carriers. Bovine serum albumin (BSA) was chosen as a
hydrophilic model protein due to its known structure and ease of quantitative
assay. BSA was encapsulated within hydroxypropyl cellulose low viscosity (HPCL)
and hydroxypropyl methyl cellulose 606 (HPMC 606) microspheres using
spray drying method, which was called by L and M formula respectively. Both
polymers have two different concentrations, low (F1L and F1M) and high
concentration (F2L and F2M). The microspheres were evaluated to FTIR, DTA,
morphology, particle size, moisture content, entrapment efficiency (EE), protein
content, yield, release profile, and aerosolization efficiency. The FTIR spectra of
protein alone and in micropsheres with polymers did not show any shift in major
peak, which indicated no protein-polymer interaction. The termogram of prepared
microspheres showed differences with polymers termogram, which indicated the
microspheres was formed. The spray drying method produced rough surfaced
microspheres with small particle size. BSA microspheres had a high EE% and
highest protein content was produced by microspheres with HPC-L polymer. The
release profile of BSA from microspheres following Higuchi kinetics models.
Aerodynamic diameters of microspheres ranged between 0,6 – 2,9 μm. The results
demonstrated this spray dried microspheres can be explored for potential protein
carriers. |
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