PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN

The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk diluent; 0,05 mg green tea...

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Bibliographic Details
Main Author: MAULINDA AGCHIRILIA VLADIKA, 061411131034
Format: Theses and Dissertations NonPeerReviewed
Language:English
Indonesian
Published: 2018
Subjects:
Online Access:http://repository.unair.ac.id/77073/1/KKC%20KK%20KH.169-18%20Vla%20p%20ABSTRAK.pdf
http://repository.unair.ac.id/77073/2/KKC%20KK%20KH.169-18%20Vla%20p%20SKRIPSI.pdf
http://repository.unair.ac.id/77073/
http://lib.unair.ac.id
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Institution: Universitas Airlangga
Language: English
Indonesian
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Summary:The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk diluent; 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Spermatozoa quality was observed first day until fifth day after diluent. The data obtained was analyzed with the analysis of variance one way ANOVA. The result showed that the percentage of motility, viability and intact plasma membrane spermatozoa at the 5th day after diluent showed a significant differece (p<0,05) between 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Motility at the 3th day after diluent respectively 46,67b ± 2,582; 43,33bc ± 4,028; 40,00ab ± 3,162; and 37,50a ± 2,739. Viability at the 3th day after diluent respectively are 79,50b ± 1,643; 77,67b ± 3,327; 77,00ab ± 3,230; and 73,83a ± 3,061. Intact plasma membrane at the 3th day after diluent respectively are 50,17b ± 2,483; 47,00ab ± 3,899; 46,83ab ± 2,858; and 46,00a ± 3,286. The conclusion of this study was the green tea extract of 0,05 mg in milk skim diluent could increase the percentage of sperm quality until 5th day after diluent.