UJI SITOTOKSISITAS BAHAN DASAR DENTIN BONDING BERBASIS 4-META DENGAN PELARUT ASETON PADA SEL FIBROBLAS PULPA GIGI
Background: Both carious and non-carious lesions which cover large dentine area are absolute indications to use dentine bonding. Clinically, dental preparation which then is subjected to composite using dentine bonding application often results in post restorative pain. Various studies suggest th...
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Format: | Theses and Dissertations NonPeerReviewed |
Language: | Indonesian Indonesian |
Published: |
2018
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Subjects: | |
Online Access: | http://repository.unair.ac.id/80914/1/PPDGS.KON.%2017-%2019%20Put%20u%20Abstrak.pdf http://repository.unair.ac.id/80914/4/PPDGS.KON.%2017-%2019%20Put%20u.pdf http://repository.unair.ac.id/80914/ http://lib.unair.ac.id |
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Institution: | Universitas Airlangga |
Language: | Indonesian Indonesian |
Summary: | Background: Both carious and non-carious lesions which cover large dentine area are
absolute indications to use dentine bonding. Clinically, dental preparation which then is subjected
to composite using dentine bonding application often results in post restorative pain. Various
studies suggest that post restorative pain is caused by the presence of residual monomers from the
imperfect polymerization of a bonding material. The residual monomer can be a free radical that
will induce oxidative stress conditions. This leads to a toxic effect on 4-META monomer as the
base material of dentine bonding. Purpose: To determine the toxic concentration of 4-META
dentine bonding material using acetone as a solvent which causes 50% death of dental pulp
fibroblast cells. Methods: Available human pulp fibroblast cells in each well, dripped with 4-
META acetone solution at concentrations 5000 μg / ml; 2500 μg / ml; 1250 μg / ml; 625 μg / ml;
312.5 μg / ml; 156.25 μg / ml; 78.12 μg / ml; 39.06 μg / ml; 19.53 μg / ml; 9.76 μg / ml and two
well were not treated as control group. The cytotoxicity test was performed with MTT-Assay Test.
The optical dencity of each well was read by Ellisa Reader and the percentage of human pulp
fibroblast cell death was calculated using the formula. Result: The concentration of 4-META
based dentine bonding with acetone solvent which can cause 50% human pulp fibroblast cell death
(LC50) is 1250 μg / ml. Conclusion: The toxic concentrations are an amount greater than or equal
to 1250 μg / ml. |
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