Expression of Osteoprotegrin and Osteoclast Level in Chronic Apical Periodontitis Induced with East Java Propolis Extract

• Main Authors: Tamara Yuanita, -, Nanik Zubaidah, -, Sri Kunarti, -
• Format: Article PeerReviewed
• Language: Indonesian; English; English; Indonesian; English; English; English
• Published: Iranian Center for Endodontic 2018
• Subjects: R Medicine; RK Dentistry
• Online Access: https://repository.unair.ac.id/84486/2/Tamara%20per%203.pdf; https://repository.unair.ac.id/84486/3/Expression%20of%20Osteoprotegrin%20and%20Osteoclast%20Level%20in%20Chronic%20Apical%20Periodontitis%20Induced%20with%20East%20Java%20Propolis%20Extract.pdf; https://repository.unair.ac.id/84486/5/5_PUBLISHED%20Iranian%20endodontic%20journal.pdf; https://repository.unair.ac.id/84486/6/04_Expression%20of%20Osteoprotegrin%20and%20Osteoclast.pdf; https://repository.unair.ac.id/84486/10/10.%20Expression.pdf; https://repository.unair.ac.id/84486/8/10.%20Expression.pdf; https://repository.unair.ac.id/84486/9/10.%20Expression.pdf; https://repository.unair.ac.id/84486/
• Institution: Universitas Airlangga

Introduction: The objective of this animal study was to promote East Java propolis as a potential natural intracanal medicament for periapical chronic apical periodontitis bone resorption through evaluating the expression of osteoprotegrin (OPG) and osteoclast level. Methods and Materials: Propolis extract was produced using a maceration procedure. Thirty Wistar rats were divided into three groups. In group I, the control group, the first upper right molar constituted a healthy tooth. In group II, containing rodents with experimentally chronicapical periodontitis, infection with Enterococcus faecalis ATCC29212 106 CFU was performed. In group III, the treatment group, after being injected with E faecalis, 10 μL propolis was applied. It required 21 days to induce post-pulp chronic apical periodontitis infection. The rats were euthanised for immunohistochemical examination in order to measure the expression of OPG and to count histologically the number of osteoclast. Result: The expression of OPG and osteoclast constituted 17.5±1.58 and 6.4±0.96 in group I, 10±2 and 16.2±1.31 in group II and 17±1.69 and 7.5±1.08 in group III. Group I presented the highest level of OPG expression but the lowest level of osteoclast expression. There were significant differences between groups II and III and group I regarding OPG and osteoclast expression (P<0.05). Conclusion: East Java Propolis was a potential intracanal medicament promoting an increase in osteoprotegerin expression and a decrease in the number of osteoclasts thereby inhibiting osteoclastogenesis.