The Effect Of Osteopontin In Semen Freezing Process On The Quality Of Holstein Friesian Dairy Bull Frozen Semen

• Main Author: Hernawati, Tatik
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Asian Academic Research Journal 2015
• Subjects: SF600-1100 Veterinary medicine
• Online Access: http://repository.unair.ac.id/84602/3/Bukti%2001.%20The%20Effect%20of%20Osteopontin%20in%20.....-min.pdf; http://repository.unair.ac.id/84602/2/Peer%20Review%20Bukti%2001.pdf; http://repository.unair.ac.id/84602/4/BUKTI%201%20THE%20EFFECT%20OF%20OSTEOPONTIN%20IN%20SEMEN%20FREEZING%20PROCESS%20ON%20THE%20QUALITY%20OF%20HOLSTEIN%20FRIESIAN%20DAIRY%20BULL%20FROZEN%20SEMEN.-compressed.pdf; http://repository.unair.ac.id/84602/; https://www.asianacademicresearch.org/2015_abstract/may_md_2015/10.pdf
• Institution: Universitas Airlangga

Background : The quality of frozen semen after thawing is one of factors in artificial insemination. Cryopreservation affects the post-thawed sperm quality. The seminal plasma diary bull’s contains high concentration of osteopontin. Our hypothesis is that osteopontin maintain sperm quality during cryopreservation. Aim : The first aim was isolation, identification and protein specification of osteopontin from dairy bull semen. The second aim was to investigate osteopontin does improve the quality of frozen semen as appears from increased expression of HSP 70 drecreased apoptotic expression and increased viability and motility. Method : isolation, identification and protein specification of osteopontin from dairy bull semen. using SDS-PAGE method indicated that there were some tapes showing different molecular weight from several samples. SDS-PAGE result from protein identification was continued with protein identification using Western Blot technique. The effect of osteopontin on the quality of frozen semen post thawing by the expression of HSP 70 with immunocytochemistry techniques. The expression of apoptotic spermatozoa by Tunnel Assay. The viability spermatozoa using negrosin eosin staining and by assesing motility of spermatozoa with direct microscopy. Result of the first phase was determined that molecular weight of osteopontin is 56 kDa. Osteopontin concentration from electroelution samples were analyzed using Nano drop method, and it showed the protein concentrations as avaregely 180 μg /ml. result of the second phase indicated that the addition of osteopontin can improve semen quality through the increase of HSP70 resulting in a decrease in apopotosis. The result of all causes increased motility mechanisms, either directly or indirectly based on viability. Conclusion : Addition of osteopontin may improve semen quality through increased HSP70 resulting in a decrease in viability and motility of apoptosis that can be maintained. Key words: Osteopontin, Friesian Holstein diary bull, seminal plasma, post- thawed sperm quality.