Penurunan Angka Fertilitas Spermatozoa dan Gambaran Histopatologis Tubulus Seminiferus Mencit (Mus Musculus) Kondisi Malnutrisi

• Main Authors: Hernawati, Tatik, Erma, Safitri, Utama, Suzanita, Mulyati, Sri
• Format: Article PeerReviewed
• Language: Indonesian; Indonesian
• Published: Fakultas Kedokteran Hewan Universitas airlangga 2012
• Subjects: SF405.5-407 Laboratory animals; SF811-909 Veterinary medicine of special organs, regions, and systems
• Online Access: http://repository.unair.ac.id/84640/1/Bukti%2013.%20Penurunan%20Angka%20Fertilitas%20Spermatozoa%20.....-min.pdf; http://repository.unair.ac.id/84640/2/Peer%20Review%20Bukti%2013.pdf; http://repository.unair.ac.id/84640/; http://journal.unair.ac.id/VETMED@decrease-of-spermatozoa-fertility-rate-and-overview-histopathologic-seminiferous-article-6023-media-28-category-5.html
• Institution: Universitas Airlangga

The research was conducted in addition aims to scientifically prove the decrease in fertility of sperm cells of male mice Mus musculus experiencing malnutrition conditions, as well as to demonstrate the histopathologic picture of the seminiferous tubules were damaged in mice Mus musculus male from malnutrition. The methods used are: Mus musculus male is experiencing malnutri ie fasted for 3 days only given to drink ad libitum compared with controls fed a normal fixed 300-400 grams per day per mice and drinking water ad libitum. We then test the fertility of sperm cells, sperm fertility rates obtained by bringing sperm and ovum cells in vitro is calculated based on the number of zygotes produced divided by the number of ova multiplied by 100%. The histopathologic examination of the seminiferous tubules of the testis is done by surgery and fabrication of histological preparations, and then observed microscopically at the damage occurs at 100 and 400 times. The results showed a significant difference between male mice created malnourished compared with controls, whereas in the treated mice showed a decrease in fertility malnutrition. Similarly, histopathologic observation of seminiferous tubules damage than the control. At the microscopic condition of malnutrition occurs in the seminiferous tubules swelling, bleeding and adhesin with the surrounding tissue and even degenerated tissue. Keywords : spermatozoa fertility rate, histopathologic seminiferous tubules, malnutrition