Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD50 parameters 1Hendrik Setia

• Main Authors: Hendrik Setia Budi, Ira Arundina, Retno Indrawati, Leonita Widyana Mahardikasari
• Format: Article PeerReviewed
• Language: English; Indonesian; English
• Published: Fakultas Kedokteran Gigi Universitas Hasanuddin 2014
• Subjects: R Medicine; RK Dentistry
• Online Access: http://repository.unair.ac.id/85305/1/394-767-1-SM.pdf; http://repository.unair.ac.id/85305/2/retno%20indra%2012.pdf; http://repository.unair.ac.id/85305/3/12ACUT1.PDF; http://repository.unair.ac.id/85305/; http://jdmfs.org/index.php/jdmfs/article/view/394
• Institution: Universitas Airlangga

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have been widely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as a potential wound healing after tooth extraction. The aim of this research was to determine the level of safety of using herbal medicine,ambonese banana stem extract on histopatology liver of mice with LD50 parameters. This experimental study was performed by the post test only controlled group design. The sample were 28 mice (Mus musculus) randomly divided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were given ambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral with sonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liver ofmice(p=0.771). Itwasconcluded that themaximumsafety dose that can still be administered is 0.42g/20gbw following theLD50 parameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showed the highest simple degeneration than all groups which were given 0.42g/20gbw dose.