Effect of hematopoietic stem cell on tumor necrosis factor- α expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice

• Main Authors: Widjiati, Suryo Kuncorojakti, Hendy Hendarto, 090315218 D, Viski Fitri Hendrawan, Aulanni'am, 090013728 D
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Wolters Kluwer-Medknow Publications 2017
• Subjects: R5-920 Medicine (General); RG1-991 Gynecology and obstetrics
• Online Access: http://repository.unair.ac.id/85356/1/Effect%20of%20hematopoietic%20stem%20cell%20on%20tumor%20necrosis%20factor-%E6%AF%A9expression%2C%20spiral%20artery%20remodeling%20and%20lacental%20apoptosis%20in%20lead-exposed%20pregnant%20mice_compressed.pdf; http://repository.unair.ac.id/85356/2/Karil%20Effect%20of%20_compressed.pdf; http://repository.unair.ac.id/85356/3/Peer%20Effect%20of%20hematopoetic.pdf; http://repository.unair.ac.id/85356/; http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=4;spage=158;epage=163;aulast=Widjiati,
• Institution: Universitas Airlangga

Objective: To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-α expression, spiral artery remodeling, and placental apoptosis. Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment; Group 2, 1/12 LD50 of lead and Aquadest; Group 3, 1/16 LD50 of lead and Aquadest, Group 4, 1/12 LD50 of lead and 1 × 107 HSC; and group 5, 1/16 LD50 of lead and 1 × 107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF-α expression and the apoptosis index. The data from all five groups were compared using the ANOVA test. Results: TNF-α expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<0.01). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the groups of 1/16 LD50 of lead followed HSC (P>0.05). Conclusions: In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF-α expression, spiral artery remodeling, and placental apoptosis index but in the group of 1/16 LD50 of lead and HSC treatment, apoptosis index has no differences with those of control group.