Expressions of growth differentiation factor-9 (gdf-9) of bovine cumulus-oocyte complex in peritoneal fluid culture of infertile patients with endometriosis

• Main Authors: Mira Myrnawati, Hendy Hendarto, 090315218 D, Lila Dewata, Widjiati
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Airlangga 2013
• Subjects: R5-920 Medicine (General); RG1-991 Gynecology and obstetrics
• Online Access: http://repository.unair.ac.id/85427/1/Expressions%20of%20Growth%20Differentation%20Factor-9%20%28GDF-9%29%20of%20Bovine%20Cumulus-Oocyte%20Complex%20in%20Peritoneal%20Fluid%20Culuture%20of%20Infertile%20Patients%20with%20Endometriosis%29.pdf; http://repository.unair.ac.id/85427/2/25.%20Expression%20of%20Growth_compressed.pdf; http://repository.unair.ac.id/85427/3/Peer%20Expressions%20of%20Growth.pdf; http://repository.unair.ac.id/85427/; http://www.journal.unair.ac.id/MOG@expressions-of-growth-differentiation-factor-9-(gdf-9)-of-bovine-cumulus-oocyte-complex-in-peritoneal-fluid-culture-of-infertile-patients-with-endometriosis-article-8228-media-57-category-3.html
• Institution: Universitas Airlangga

The decrease of GDF-9 production may impair oocyte granulosa cell communication and lead to failure of fertilization and embryonic development. The objective of this study was to study expression of growth differentiation factor-9 (GDF-9) cumulus-oocyte complex in peritoneal culture of infertile patients with endometriosis. Peritoneal fluid were collected from infertile patients undergoing diagnostic laparoscopy and classified into non-endometriosis, mild endometriosis, and severe endometriosis according to laparoscopic finding. Cumulus-oocyte complex (COC) were aspirated from antral follicle derived from bovine ovary from local abattoirs. COC were then placed in tissue culture medium-199 (TCM-199) and divided into four group of cultere. First group contain TCM-199 as a medium served as control group, while second, third, and four groups medium were added accordingly with 3% of peritoneal fluid of non endometriosis, mild endometriosis, and severe endometriosis. These four groups were incubated in 5% CO2 at 38oC for 20 hours and COC were eventually fixated in object glasses, stained by immunocytochemistry, and followed by measurement of GDF-9 expression. Statistic analysis using Kruskal Wallis test showed differences of GDF-9 expression in four culture groups. Mann-Whitney test revealed that GDF-9 expression in endometriosis group (mild and severe) were significantly lower compared with control and non-endometriosis group and expression in severe endometriosis group were significantly lower than in mild endometriosis one (p < 0.05). In conclusion,GDF-9 expressions of bovine COC cultured in peritoneal fluid of infertile patients with endometriosis were lowered compared with that of control and non-endometriosis group and this decrease was in proportion with severity of endometriosis.(MOG 2013;21:71-76)